广东省部分地区禽源和猪源沙门菌血清型与耐药性及Ⅰ类整合子分析

为了解广东省禽场和猪场沙门菌的血清型和耐药性情况,本研究2015年从广东省多个养殖场共采集样品126份,分离出沙门菌24株,分离率为19.04%。采用Kauffmann-White法、Kirby-Bauer法和PCR方法对分离株进行了血清型鉴定、药敏试验和Ⅰ类整合子检测。血清型鉴定结果显示,24株沙门菌共鉴定出4种血清型,分别是鼠伤寒沙门菌(14株)、印第安纳沙门菌(8株)、科瓦利斯沙门菌(1株)和阿尔巴尼沙门菌(1株)。药敏试验结果显示,分离株对四环素(83.33%)、氨苄西林(70.83%)、磺胺异恶唑(70.83%)、萘啶酸(66.67%)、复方新诺明(58.33%)、卡那霉素(54.17%)、阿米卡星(54.17%)、庆大霉素(50.00%)的耐药率较高,有54.17%(13/24)的菌株对8种及8种以上抗菌药物耐药。Ⅰ类整合子检测结果显示,Ⅰ类整合酶阳性率为29.17%(7/24),整合酶阳性菌株中仅有1株扩增到携带耐药基因aadA2的基因盒。上述结果表明,广东省禽场和猪场沙门菌分离率较高,耐药情况严重,菌株的耐药性与其血清型和Ⅰ类整合子的携带有一定的相关性。

Analysis on Serotypes,Drug Resistance and ClassⅠ Integron of Salmonella from Poultry and Swine Sources in Some Areas of Guangdong Province

In order to understand the serotypes,antimicrobial resistance and the prevalence of classⅠintegron of Salmonella isolates,126 animal samples were collected from poultry farms and pig farms in Guangdong province in 2015.Twenty-four (19.04%) Salmonella strains were isolated from the samples.The Kauffmann-White method,the Kirby-Bauer test and PCR were used respectively for serotype identification,drug susceptibility test and classⅠintegron test.In result,serotype identification showed that 4 Salmonella serotypes were detected,including S.typhimurium (14),S.indiana (8),S.corvallis (1),S.albany (1).Among 24 Salmonella strains,83.33%,70.83%,70.83%,66.67%,58.33%,58.33%,54.17%,54.17% and 50.00% were resistant to tetracycline,ampicillin,sulfisoxazole,nalidixic acid,cotrimoxazole,kanamycin,amikacin and gentamicin,respectively,and displayed different resistance rates to the rest drugs.54.17%(13/24) of the isolates resistant to eight or more antimicrobials.29.17%(7/24) of the isolates were positive for intI,and only one isolate carry resistance gene cassette(aadA2).These results indicated that Salmonella has a high isolation rate in farms of Guangdong and performs serious drug resistance which is related to the serotypes and classⅠintegron to a certain extent.