大庆市及周边地区非免疫猪群猪圆环病毒感染情况调查

为了解大庆市及周边地区猪群中PCV的感染情况,应用ELISA和PCR技术对采自大庆市5区4县及周边地区55个村屯/猪场未免疫PCV2疫苗猪血清样本649份,5家病猪场和6家屠宰场组织样本448套,进行血清学和分子生物学检查,并对检测结果进行了比较分析。结果显示,大庆市及周边地区未免疫PCV2疫苗猪血清样品PCV2抗体平均阳性率为54.70%,其中断奶仔猪样品阳性率为50.68%,育肥猪样品为70.00%,繁育母猪样品为54.70%。组织样品PCV1阳性率为4.46%,PCV2阳性率为22.32%。调查结果可为确定地区流行毒株序列提供原始材料和制定科学防控措施提供依据。

Study on PCV Infection Situation of Non Immune Pigs in Daqing City and Surrounding Areas

The experiment was conducted to detect the infection situation of PCV in pigs in Daqing city and surrounding areas.649 serum samples from 55 villages or pig farms (non-immune pigs) were used to test PCV2 antibodies by enzyme-linked immunosorbent assay (ELISA), and the 488 tissue samples from 5 sick-pig farms and 6 slaughterhouses (PCV2 immune pigs) were used to test PCV1 or PCV2 by polymerase chain reaction (PCR).The results showed that the average positive rate of PCV2 antibodies was 54.70% in the PCV2 non-immune pigs, including the positive rates of sera of weaned piglets, finishing pigs and breeding sows with 50.68%, 70.00% and 54.70%, respectively.The positive rate of PCV1 in tissues of pigs was 4.46%, that of PCV2 in tissues of pigs was 22.32%.The survey was likely to determine pandemic strain sequence and provide evidences for taking scientific prevention in Daqing area.