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| 猪伪狂犬病病毒PCR检测方法的建立与应用 | |
| 引用本文： | 吴发兴,郑辉,亓传德,高许雷,张志,刘爽,张燕霞,李晓成.猪伪狂犬病病毒PCR检测方法的建立与应用[J].动物医学进展,2009,30(5). |
| 作者姓名： | 吴发兴 郑辉 亓传德 高许雷 张志 刘爽 张燕霞 李晓成 |
| 作者单位： | 1. 中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032;东北农业大学,黑龙江哈尔滨,150030 3. 中国动物卫生与流行病学中心,山东青岛,266032;青岛农业大学,山东青岛,266109 |
| 摘 要： | 参考GenBank中收录的猪伪狂犬病病毒gE基因序列,应用Primer5.0软件设计了一对引物,扩增目的片段为632 bp.进行PCR检测方法的特异性、敏感性、重复性及符合性试验,建立了PRV的PCR检测方法.应用该方法对临床疑似发病样品进行了检测,PRV检出率为14.6%.表明该方法具有快速、特异、敏感、重复性好等优点,可用于PRV的临床发病诊断及流行病学监测等. |
| 关 键 词： | 猪伪狂犬病病毒 gE基因 聚合酶链式反应 |
Establishment and Application of PCR for Detection of Porcine Pseudorabies virus |
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| WU Fa-xing,ZHENG Hui,QI Chuan-de,GAO Xu-lei,ZHANG Zhi,LIU Shuang,ZHANG Yan-xia,LI Xiao-cheng.Establishment and Application of PCR for Detection of Porcine Pseudorabies virus[J].Progress In Veterinary Medicine,2009,30(5). | |
| Authors: | WU Fa-xing ZHENG Hui QI Chuan-de GAO Xu-lei ZHANG Zhi LIU Shuang ZHANG Yan-xia LI Xiao-cheng |
| Institution: | 1.China Animal Health and Epidemiology Center;Qingdao;Shandong;266032;China;2.Northeast Agricultural University;Harbin;Heilongjiang;150030;3.Qingdao Agricultural University;266109;China |
| Abstract: | According to the gE genomic sequence of PRV published in GenBank,and using the Primer5.0 software,a pair of primers were designed,the motive fragment of amplification is 632bp.A PCR detection method was established by specificity,sensibility,reproducibility and parallelism tests,The method had some advantages such as fast,specific,sensitive and repeatable.And it was considered to be the foundation for detectein and epidemiologic survey of PRV.Using the method to detect the clinic samples,the positive rate w... |
| Keywords: | Porcine pseudorabies virus gE gene PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |