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| 鸡传染性法氏囊病病毒XX08株VP2高变区基因的克隆与序列分析 | |
| 引用本文： | 岳锋,宁红梅,银梅,葛亚明,朱彦彩,周娟娟,贾文科,王文强,黄小东,王选年.鸡传染性法氏囊病病毒XX08株VP2高变区基因的克隆与序列分析[J].动物医学进展,2010,31(3):35-39. |
| 作者姓名： | 岳锋 宁红梅 银梅 葛亚明 朱彦彩 周娟娟 贾文科 王文强 黄小东 王选年 |
| 作者单位： | 1. 河南科技学院,动物科学学院,河南新乡,453003 2. 双汇集团养殖部饲料厂,河南漯河,462000 3. 河南科技大学动物科技学院,河南洛阳,471003 4. 河南科技学院,动物科学学院,河南新乡,453003;新乡学院,河南新乡,453003 |
| 基金项目： | 国家自然科学基金项目(30871885) |
| 摘 要： | 应用反转录(RT-PCR)技术从河南新乡某鸡场分离的鸡传染性法氏囊病病毒XX08株总RNA中克隆出593bp的VP2高变区基因。序列分析表明,XX08毒株VP2高变区氨基酸序列与欧洲超强毒株UK661、日本超强毒株OKYM以及经典弱毒株D78的同源性均为96.8%。遗传进化分析表明,该毒株与超强毒株UK661和OKYM位于同一进化树。XX08毒株与超强毒株具有相似的氨基酸特征,但是222位的A被P替代,提示XX08毒株可能为中等强毒力毒株。 |
| 关 键 词： | 传染性法氏囊病病毒 VP2 克隆 |
Cloning and Analysis of Highly Variable Region VP2 Gene in Infectious bursal disease virus XX08 Strain |
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| YUE Feng,NING Hong-mei,YIN Mei,GE Ya-ming,ZHU Yan-cai,ZHOU Juan-juan,JIA Wen-ke,WANG Wen-qiang,HUANG Xiao-dong,WANG Xuan-nian.Cloning and Analysis of Highly Variable Region VP2 Gene in Infectious bursal disease virus XX08 Strain[J].Progress In Veterinary Medicine,2010,31(3):35-39. | |
| Authors: | YUE Feng NING Hong-mei YIN Mei GE Ya-ming ZHU Yan-cai ZHOU Juan-juan JIA Wen-ke WANG Wen-qiang HUANG Xiao-dong WANG Xuan-nian |
| Institution: | 1.College of Animal Sciences;Henan Institute of Science and Technology;Xinxiang;Henan;453003;China;2.Animal Feed Factory of Cultivation Department of Shuanghui Clique;Luohe;462000;3.College of Animal Sciences;Henan University of Science and Technology;Luoyang;471003;4.Xinxiang University;China |
| Abstract: | The Infectious bursal disease virus(IBDV)XX08 strain was isolated from a chicken farm in Xinxiang city of Henan province.The highly variable region VP2 gene,593 bp,was cloned from the total RNA of the XX08 strain by RT-PCR.Amino acid sequence analysis showed that there was 96.8% homology among the European vvIBDV strain of OKYM,Japanese UK661,classical attenuated strain D78 and the isolated strain of XX08.Compared with the highly variable region of these strain,the amino acid residue A in 222 position of po... |
| Keywords: | Infectious bursal disease virus VP2 cloning |
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