| 藏系绵羊IGF-1基因表达水平的实时荧光定量PCR检测 |
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| 引用本文： | 窦全林,杨发龙,王杰,钟金诚,郑玉才,陈刚.藏系绵羊IGF-1基因表达水平的实时荧光定量PCR检测[J].动物医学进展,2008,29(7). |
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| 作者姓名： | 窦全林 杨发龙 王杰 钟金诚 郑玉才 陈刚 |
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| 作者单位： | 1. 青海大学农牧学院动物医学系,青海西宁,810003
2. 西南民族大学生命科学与技术学院,四川成都,610041 |
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| 基金项目： | 青海省科技厅资助项目
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| 摘 要： | 采用实时荧光定量PCR SYBR GreenI荧光染料法,对藏系绵羊皮肤细胞中IGF-1基因的表达水平进行了相对定量分析,建立了快速检测IGF-1基因表达量的方法。通过该方法检测出了IGF基因在藏系绵羊皮肤细胞中的相对表达量,且表达量有差异。该方法具有灵敏度高、重复性好、定量准确、速度快等优点。
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| 关 键 词： | 实时荧光定量PCR 基因IGF-1 表达 藏系绵羊 |
Detection of Expression Level of IGF-1 Gene by Real-time Quantitative PCR in Tibetan Sheep |
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| DOU Quan-lin,YANG Fa-long,WANG Jie,ZHONG Jin-cheng,ZHENG Yu-cai,CHEN Gang.Detection of Expression Level of IGF-1 Gene by Real-time Quantitative PCR in Tibetan Sheep[J].Progress In Veterinary Medicine,2008,29(7). |
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| Authors: | DOU Quan-lin YANG Fa-long WANG Jie ZHONG Jin-cheng ZHENG Yu-cai CHEN Gang |
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| Abstract: | The expression levels of IGF-1 from Tibetan sheep skin cells were relatively analysised by using fluorescent quantitative PCR SYBR Green I fluorescent dye technology,and the methods for rapid detecting the expression of IGF-1 gene approach were established.The relative expression level of IGF-1 in Tibetan sheep skin cells was detected by using this method.The results showed that the expressions were different.The method has many advantages,such as high sensitivity,reproducibility,quantitative accuracy,rapid speed and other characteristics. |
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| Keywords: | real-time quantitative PCR gene expression level Tibetan sheep |
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