| 猪伪狂犬病病毒检测方法研究进展 |
| |
| 引用本文： | 郭广君,吕素芳,肖跃强,毕研丽,魏凤,沈志强,丁壮.猪伪狂犬病病毒检测方法研究进展[J].动物医学进展,2011(12):84-88. |
| |
| 作者姓名： | 郭广君 吕素芳 肖跃强 毕研丽 魏凤 沈志强 丁壮 |
| |
| 作者单位： | 吉林大学畜牧兽医学院;山东省滨州畜牧兽医研究院预防兽医学与动物生物技术重点实验室;山东绿都生物科技有限公司 |
| |
| 基金项目： | 滨州市应用技术研究与开发专项基金(200706);山东省滨州畜牧兽医研究院青年科技创新基金(2007-02) |
| |
| 摘 要： | 随着免疫学与分子生物学技术日臻完善,针对猪伪狂犬病的血清学方法和分子生物学检测方法不断改进.论文就近年来针对猪伪狂犬病病毒的中和试验、酶联免疫吸附试验、乳胶凝集试验、胶体金免疫层析、核酸探针、基因芯片、多重PCR、LAMP等检测方法研究概况做一综述,为寻找合适的快速准确的检测方法、制定合理的免疫程序和发展新型的检测方法...
|
| 关 键 词： | 猪伪狂犬病病毒 检测方法 基因芯片 实时定量PCR 环介导等温扩增 |
Progress on Detection Method of Pseudorabies Virus |
| |
| GUO Guang-jun,LV Su-fang,XIAO Yue-qiang,BI Yan-li,WEI Feng,SHEN Zhi-qiang,DING Zhuang.Progress on Detection Method of Pseudorabies Virus[J].Progress In Veterinary Medicine,2011(12):84-88. |
| |
| Authors: | GUO Guang-jun LV Su-fang XIAO Yue-qiang BI Yan-li WEI Feng SHEN Zhi-qiang DING Zhuang |
| |
| Institution: | 1 (1.The College of Animal Husbandry and veterinary of Jilin University,Changchun,Jilin,130062,China; 2.Shandong Binzhou Animal Science & Veterinary Medicine Insitute,Binzhou,Shandong,256600,China; 3.Shandong Lvdu Bio-industry Company,LTD.,Binzhou,Shandong,256600,China) |
| |
| Abstract: | With development of the immunology and molecular biology technology,conventional serologic methods and molecular biological detection methods are constantly emerging for pseudorabies.In this paper,a review for the swine pseudorabies tests by neutralization test,enzyme-linked immunosorbent assay,latex agglutination test,colloidal gold immunochromatography,nucleic acid probes,gene chip,multiple PCR,LAMP and other detection methods in recent years were summarized.The aim is to find a suitable,rapid and accurate diagnostic method and provide references for making rational immune programmes and developing new diagnostic methods. |
| |
| Keywords: | Pseudorabies virus detection method gene chip real-time quantitative PCR loop-mediated isothermal amplification |
| 本文献已被 CNKI 等数据库收录！ |
|
