| 细胞凋亡的主要检测方法研究进展 |
| |
| 引用本文： | 张广智,胡长敏,陈颖钰,刘颖,李帅,罗雨,王金勇,郭爱珍,陈焕春.细胞凋亡的主要检测方法研究进展[J].动物医学进展,2011(8):89-92. |
| |
| 作者姓名： | 张广智 胡长敏 陈颖钰 刘颖 李帅 罗雨 王金勇 郭爱珍 陈焕春 |
| |
| 作者单位： | 华中农业大学动物医学院;华中农业大学农业微生物学国家重点实验室;国家肉牛产业技术体系疾病控制功能研究室; |
| |
| 基金项目： | 现代农业(肉牛)产业技术体系(nycytx-38)建设专项资金资助; 公益性行业(农业)科研专项基金资助(201003060); 华中农业大学自主创新基金项目资助(2010QC002) |
| |
| 摘 要： | 细胞凋亡是生理性细胞死亡过程,凋亡在维持动物机体内环境平衡中扮演着极其重要的角色.细胞凋亡的准确检测是研究细胞凋亡相关机制,以及评价细胞凋亡在相应组织或疾病中影响的第一步.形态学是检测细胞凋亡的最直观的方法,至今一直作为细胞凋亡检测的金标准.DNA片段化检测主要有DNA ladder,TUNEL检测法和ELISA检测法...
|
| 关 键 词： | 细胞凋亡 线粒体膜电位 流式细胞术 |
Recent Advance on the Ways to Apoptosis Detection |
| |
| ZHANG Guang-zhi,HU Chang-min,CHEN Ying-yu,LIU Ying,LI Shuai,LUO Yu,WANG Jin-yong,GUO Ai-zhen,CHEN Huan-chun.Recent Advance on the Ways to Apoptosis Detection[J].Progress In Veterinary Medicine,2011(8):89-92. |
| |
| Authors: | ZHANG Guang-zhi HU Chang-min CHEN Ying-yu LIU Ying LI Shuai LUO Yu WANG Jin-yong GUO Ai-zhen CHEN Huan-chun |
| |
| Institution: | ZHANG Guang-zhi1,2,HU Chang-min1,3,CHEN Ying-yu1,LIU Ying1,LI Shuai1,LUO Yu1,WANG Jin-yong1,GUO Ai-zhen1,CHEN Huan-chun1,2(1.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,Huber,430070,China,2.The Stated Key Laboratory of Agricultural Microbiology,3.National beef Cattle Industrial Technology System,China) |
| |
| Abstract: | Apoptosis is a programmed,physiological mode of cell death that plays an important role in maintaining the homeostasis of internal environment.The accurate detection of apoptosis is the first step to analysis the relative mechanisms of apoptosis and to assess the impact of apoptosis on the respective tissue and the disease.Morphologic is the most intuitionistic method to detect the cell apoptosis,and is still considered to be golden standard.DNA ladder,TUNEL,and ELISA are used to detect the DNA fragmentatio... |
| |
| Keywords: | cell apoptosis mechanism mitochondria membrane potential flow cytometry |
| 本文献已被 CNKI 等数据库收录！ |
|
