| 禽流感病毒NS1蛋白亚细胞定位研究 |
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| 引用本文： | 朱春玉,孙婷婷,郑方亮,艾海新,朱俊丰,王宁,商玲玲,刘宏生.禽流感病毒NS1蛋白亚细胞定位研究[J].动物医学进展,2011(10):10-14. |
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| 作者姓名： | 朱春玉 孙婷婷 郑方亮 艾海新 朱俊丰 王宁 商玲玲 刘宏生 |
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| 作者单位： | 辽宁大学生命科学院辽宁省高校动物资源与疫病防治重点实验室; |
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| 基金项目： | 国家自然科学基金项目(30671852); 病毒学国家重点实验室开放研究基金项目(2010009); 辽宁省百千万人才工程项目(022064); 辽宁省教育厅重点实验室项目(2009S043); 辽宁大学青年科研基金项目(488020) |
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| 摘 要： | 为深入研究NS1蛋白的生物学功能,了解NS1蛋白在哺乳动物细胞中的分布和亚细胞定位情况.采用RT-PCR扩增出禽流感病毒NS1基因片段,克隆至载体pEGFP-N1,经酶切和测序鉴定后,将构建的重组质粒pEGFP-N1-NS1经脂质体介导转染293T细胞和Hela细胞,在荧光显微镜和激光扫描共聚焦显微镜下观察NS1在细胞...
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| 关 键 词： | 禽流感病毒 NS1蛋白 亚细胞定位 |
Subcellular Localization of Avian Influenza Virus NS1 Protein |
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| ZHU Chun-yu,SUN Ting-ting,ZHENG Fang-liang,AI Hai-xin,ZHU Jun-feng,WANG Ning,SHANG Ling-ling,LIU Hong-sheng.Subcellular Localization of Avian Influenza Virus NS1 Protein[J].Progress In Veterinary Medicine,2011(10):10-14. |
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| Authors: | ZHU Chun-yu SUN Ting-ting ZHENG Fang-liang AI Hai-xin ZHU Jun-feng WANG Ning SHANG Ling-ling LIU Hong-sheng |
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| Institution: | ZHU Chun-yu,SUN Ting-ting,ZHENG Fang-liang,AI Hai-xin,ZHU Jun-feng,WANG Ning,SHANG Ling-ling,LIU Hong-sheng(Key Laboratory of Animal Resource and Epidemic Disease Prevention,Life Science School of Liaoning University,Shenyang,Liaoning,110036,China) |
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| Abstract: | In order to deeply study on biological function of NS1 protein,learn about distribution and subcellular localization of NS1 protein in mammalian cells.NS1 gene of avian influenza virus was amplified by RT-PCR,and cloned into vector pEGFP-N1.Recombinant plasmid was identified by enzyme digestion and sequencing,the recombinant plasmid pEGFP-N1-NS1 was transfected into 293T cells and Hela cells by liposome,distribution and subcellular localization of NS1 protein was observed by the fluorescence microscope and ... |
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| Keywords: | Avian influenza virus NS1 protein subcellular localization |
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