| rhEPO对缺糖缺氧大鼠星形胶质细胞GLT-1和GLAST表达的影响 |
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| 引用本文： | 庞一强,杨静,吴刚,汪静,姜树原.rhEPO对缺糖缺氧大鼠星形胶质细胞GLT-1和GLAST表达的影响[J].动物医学进展,2016(10):81-84. |
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| 作者姓名： | 庞一强 杨静 吴刚 汪静 姜树原 |
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| 作者单位： | 1. 包头市第四医院,内蒙古包头,014030;2. 包头医学院基础医学与法医学院,内蒙古包头,014060;3. 包头医学院基础医学与法医学院，内蒙古包头 014060; 包头医学院中心实验室，内蒙古包头 014060 |
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| 基金项目： | 内蒙古自治区自然科学基金项目(2013MS1111)；包头市医药卫生科技计划项目(Wsjj2015016) |
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| 摘 要： | 为了研究重组人促红细胞生成素(rhEPO)对缺糖缺氧(OGD)培养大鼠星形胶质细胞GLT-1和GLAST表达的影响,将缺糖缺氧培养星形胶质细胞分成不同浓度rhEPO处理组:0、20、100U/mL,不同浓度rhEPO与星形胶质细胞在缺氧缺糖条件下培养6h,用RT-PCR测定GLT-1和GLAST的mRNA表达变化,免疫印迹技术测定GLT-1和GLAST蛋白的表达变化。20、100U/mL rhEPO星形胶质细胞GLT-1的mRNA和蛋白质水平较OGD对照组明显升高(P0.05),GLAST的mRNA和蛋白质水平变化不明显(P0.05)。GLT-1水平可能与rhEPO对缺糖缺氧培养大鼠星形胶质细胞的保护作用有关。
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| 关 键 词： | 重组人促红细胞生成素 星形胶质细胞 GLT-1 GLAST 缺糖缺氧 |
Effects of rhEPO on Expressions of GLT-1 and GLAST in Rat Astrocyte of Cultured by Oxygen-glucose Deprivation |
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| Abstract: | In order to study effects of rhEPO on the expressions of GLT-1 and GLAST in rat astrocytes cul-tured by oxygen-glucose deprivation,the astrocytes of rats cultured by oxygen-glucose deprivation were di-vided into three groups with different concentrations of rhEPO 0,20,100 U/mL and cultured for 6 hours by hypoxia-glucose deprivation.The real-time PCR and Western blot were used to detect the changes of mRNA and protein expressions of GLT-1 and GLAST,respectively.In comparison with OGD control,mR-NA and protein levels of GLT-1 were found to be increased in the groups of 20 and 100 U/mL rhEPO (P <0.05),but the changes of GLAST were not obvious(P >0.05).The changes of GLT-1 may be related to protective effects of rhEPO on astrocytes of rats cultured by oxygen-glucose deprivation. |
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| Keywords: | rhEPO astrocyte GLT-1 GLAST oxygen-glucose deprivation |
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