The assessment of genetic diversity within and among the eight subpopulations of Japanese Black cattle using 52 microsatellite markers |
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| Authors: | Takahiro Nishimaki Takayuki Ibi Yoshihiro Tanabe Yoshiyuki Miyazaki Naohiko Kobayashi Tamako Matsuhashi Takayuki Akiyama Emi Yoshida Kazumi Imai Mayu Matsui Keiichi Uemura Naoto Watanabe Tatsuo Fujita Yosuke Saito Tomohiko Komatsu Takahisa Yamada Hideyuki Mannen Shinji Sasazaki Tetsuo Kunieda |
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| Institution: | 1. Graduate School of Natural Science and Technology, Okayama University, , Okayama, Kagawa, Japan;2. Graduate School of Environmental and Life Science, Okayama University, , Okayama, Kagawa, Japan;3. Maebashi Institute of Animal Science, Livestock Improvement Association of Japan, Inc., , Maebashi, Kagawa, Japan;4. Gifu Prefectural Hida Region Livestock Hygiene Service Center, , Takayama, Kagawa, Japan;5. Gifu Prefectural Livestock Research Institute, , Takayama, Kagawa, Japan;6. Northern Center of Agricultural Technology, General Technological Center of Hyogo Prefecture for Agriculture, Forest and Fishery, , Asago, Kagawa, Japan;7. Health and Environment Center, Hiroshima Prefectural Technology Research Institute, , Hiroshima, Kagawa, Japan;8. Livestock Technology Research Center, Hiroshima Prefectural Technology Research Institute, , Shobara, Kagawa, Japan;9. Kagawa Prefecture Livestock Experiment Station, , Miki, Kagawa, Japan;10. Livestock Research Division, Oita Prefectural Agriculture, Forestry and Fisheries Research Center, , Taketa, Niigata, Japan;11. Miyagi Prefectural Livestock Experiment Station, , Ohsaki, Niigata, Japan;12. Agricultural Research Center, Yamagata Prefectural Animal Industrial Institute, , Shinjo, Niigata, Japan;13. Department of Agrobiology, Faculty of Agriculture, Niigata University, , Nishi, Niigata, Japan;14. Graduate School of Agricultural Science, Kobe University, , Nada, Kobe, Japan |
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| Abstract: | Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise FST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies. |
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| Keywords: | genetic diversity Japanese Black cattle microsatellite marker |
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