The genetic organization of the capsular polysaccharide biosynthesis region
of Actinobacillus pleuropneumoniae serotype 14 |
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| Authors: | Hiroya ITO |
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| Institution: | 1)National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1–5 Kannondai, Tsukuba, Ibaraki, Japan |
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| Abstract: | The genetic organization of the gene involved in the capsular polysaccharide
(CPS) biosynthesis of Actinobacillus pleuropneumoniae serotype 14 has
been determined. The DNA region for the CPS biosynthesis of serotype 14
(cps14) comprised 9 open reading frames, designated as
cps14AB1B2B3CDEFG genes, encoding
Cps14A to Cps14G protein, respectively. Cps14A was similar to CpsA of A.
pleuropneumoniae serotypes 1, 4 and 12; the Cps14B1 and
Cps14B2 were similar to CpsB of A. pleuropneumoniae
serotypes 1, 4 and 12, suggesting that CPS structure of A.
pleuropneumoniae serotype 14 would belong to Group I including A.
pleuropneumoniae serotypes 1, 4, 12 and 15. Surprisingly, the overall
nucleotide sequence, deduced amino acid sequence, and the genetic organization of the
cps14 were nearly identical to those of Actinobacillus
suis. This study will provide the molecular basic knowledge for development of
diagnostics and vaccine of A. pleuropneumoniae serotype 14. |
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| Keywords: | Actinobacillus pleuropneumoniae serotype 14 Actinobacillus suis capsular polysaccharide biosynthesis genetic organization |
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