The effects analysis of two neonicotinoid insecticides on in
vitro maturation of porcine oocytes using hanging drop monoculture
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Authors: | Sadamasa ISHIKAWA Kou HIRAGA Yuuki HIRADATE Kentaro TANEMURA |
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Institution: | 1)Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981–8555, Japan |
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Abstract: | Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong
affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect
control by hyperstimulating insect nerves and are used for agricultural pest management.
However, it has also been reported that ACE and IMI affect mammalian reproductive
function. We determined the effects of ACE and IMI on the in vitro
maturation of porcine oocytes. Significant decreases in nuclear maturation rates were
observed in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE or
IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and
IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on
the concentration of exposure. |
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Keywords: | hanging drop in vitro maturation (IVM) neonicotinoid porcine oocyte |
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