Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a
synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen |
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| Authors: | Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Ken MAEDA Takashi KONDO |
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| Institution: | 1)Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan;2)Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan |
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| Abstract: | To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4
(EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) 4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer:
MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more
strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera
from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%),
whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer
ELISA. |
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| Keywords: | EHV-4 glycoprotein G peptide ELISA |
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