Rapid detection of contagious ecthyma by loop-mediated isothermal
amplification and epidemiology in Jilin Province China |
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Authors: | Kai WANG Hongze SHAO Zhihua PEI Guixue HU |
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Institution: | 1)College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.2888, Changchun 130118, P.R. China;2)Department of Preventive Veterinary Medicine, Jilin Academy of Animal Science and Veterinary Medicine, Xi’an Road No.4510, Changchun, 130062, P.R. China |
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Abstract: | The aim of this experiment was to develop a loop-mediated isothermal amplification (LAMP)
assay and to research the recent epidemiology of contagious ecthyma in Jilin Province,
China, using the assay. A LAMP assay targeting a highly conserved region of the F1L gene
was developed to detect contagious ecthyma virus (CEV). Three hundred and sixty-five cases
from 64 flocks in 9 different areas of Jilin Province, China, from 2011 to 2014 were
tested using the LAMP assay. The results showed that the sensitivity of the LAMP assay was
100 copies of the standard plasmid, which is 100-fold higher than the sensitivity of PCR.
No cross-reactivity was observed with capripoxvirus, fowlpox virus, foot-and-mouth disease
virus serotype O, foot-and-mouth disease virus serotype Asia I and bluetongue virus. The
average positive rate was 19.73% (72/365), and the positive rate was highest in lambs aged
1–6 months. Our results demonstrated that CEV infection was very widespread in the flocks
of Jilin Province and that the LAMP assay allows for easy, rapid, accurate and sensitive
detection of CEV infection. |
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Keywords: | contagious ecthyma virus (CEV) epidemiology loop-mediated isothermal amplification (LAMP) |
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