伊犁马对补喂α-酮异己酸的吸收及其某些相关代谢的研究

为探究伊犁马对补喂不同水平α-酮异己酸的吸收及其某些相关代谢,采取单因素多水平试验设计方法,选取20匹年龄为1岁,体重(295.25±25.15)kg的伊犁马,分成4组,每组5匹。试验Ⅰ、Ⅱ、Ⅲ组分别补喂6、12、18 gα-酮异己酸,进行为期25 d的试验。在试验第25天采集血液样品。结果表明:(1)随着α-酮异己酸补喂水平的增加,血浆中α-酮异己酸浓度的变化呈先上升后下降的趋势。与对照组相比,补喂α-酮异己酸1 h后各试验组血浆α-酮异己酸浓度分别提高了11.2%、7.86%和21.51%,且均达到了峰值(P>0.05);(2)随着α-酮异己酸补喂水平的增加,血浆中β-羟基-β-甲基丁酸浓度的变化呈先下降后上升再下降的趋势。与对照组相比,各试验组补喂前0 h血浆β-羟基-β-甲基丁酸浓度呈下降趋势,补喂后1、2、4 h血浆β-羟基-β-甲基丁酸浓度均有所升高(P>0.05);(3)与对照组相比,各试验组血浆α-酮异己酸和β-羟基-β-甲基丁酸浓度均差异不显著(P>0.05);试验Ⅱ组和试验Ⅲ组血浆亮氨酸浓度分别提高了22.40%和22.24%(P>0.05);试验Ⅰ组和试验Ⅲ组血浆异亮氨酸浓度极显著降低(P<0.01);血浆缬氨酸浓度显著降低(P<0.05);试验Ⅲ组血浆赖氨酸、甲硫氨酸、精氨酸和谷氨酰胺浓度均显著降低(P<0.05);各试验组血浆5-羟色胺浓度极显著降低(P<0.01);试验Ⅲ组血浆三甲基组氨酸浓度降低了18.74%(P>0.05)。补喂α-酮异己酸提高了伊犁马血浆α-酮异己酸浓度,且各试验组均在补喂后1 h达到峰值;提高了血浆β-羟基-β-甲基丁酸和亮氨酸的浓度。通过补喂不同水平的α-酮异己酸,改变了伊犁马对某些氨基酸的吸收利用。

Study on the absorption and some related metabolism ofα-ketoisocaproic in Yili horse

The aim of the experiment was to study Yili horse's absorption of diet supplementated with different levels of dose ofα-ketoisocaproic(KIC)and its related metabolism.Single factor multilevel test design were used,twenty Yili horses with similar body weight(295.25±25.15)kg]and aged 1 year old,were divided into 4 groups,with 5 horses in each group.Trial groupsⅠ,Ⅱ,Ⅲwere supplemented 6,12,18 g KIC,respectively.The experiment period was 25 days.Blood samples were collected on experiment 25 d.The results showed that:(1)With the increase of KIC feeding level,the change of KIC concentration in plasma first increased and then decreased.When the KIC feeding level reached a certain amount,the absorption of KIC by Yili horse was saturable.Compared with control group,complementary feed each trial group after 1 h,the plasma KIC concentration was increased by 11.2%,7.86%and 21.51%,respectively,and reached a peak(P>0.05).(2)With the increase of KIC feeding level,the change of HMB concentration in plasma showed a trend of decreasing first,then increasing and then decreasing.Compared with the control group,the plasma HMB concentration in each trial group decreased at 0 h before supplementary feeding,and increased at 1,2,4 h after supplementary feeding(P>0.05).(3)Compared with the control group,the plasma KIC and HMB concentration of each trial group were not significantly different(P>0.05).The plasma leucine concentration of trial groupⅡand trial groupⅢwas increased by 22.40%and 22.24%respectively,but the difference was not significant(P>0.05).Trial groupⅠand trial groupⅢof plasma isoleucine concentration significantly lower(P<0.01),and the plasma concentration of valine was significantly decreased(P<0.05).The lysine,methionine,arginine and glutamine levels in plasma of trial groupⅢwere significantly lower(P<0.05).The serotonin levels of plasma in each experimental group were significantly decreased(P<0.01).The three methyl histidine concentration in plasma of trial groupⅢwas reduced by 18.74%(P>0.05).KIC supplemention increased the plasma KIC concentration of Yili horse,and the peak value was 1 h after feeding in each experimental group.The concentration of HMB and leucine in plasma were increased.By feeding different levels of KIC,the absorption and utilization of some amino acids in Yili horse were changed.