超高效液相色谱法测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量方法的研究

为有效快速测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量,饲料经甲醇、正己烷和乙酸乙酯混合液萃取后,在氮气保护下浓缩,用甲基叔丁基醚-乙腈溶液复溶,注入超高效液相色谱仪中进行测定。结果表明:斑蝥黄浓度为1.25~10.0μg/mL,β-阿朴-8’-胡萝卜素酸乙酯为2.50~20.0μg/mL线性关系良好;斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯的检测限为0.06 mg/kg,定量限为0.20 mg/kg。斑蝥黄5个添加水平的平均回收率为72.8%~82.8%,相对标准偏差(RSD)为0.41%~1.06%;β-阿朴-8’-胡萝卜素酸乙酯5个添加水平的平均回收率为72.0%~82.8%,RSD为0.46%~1.41%。说明该方法简便、快速、灵敏度高、准确度好,适用于测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量。

Study on the determination of canthaxanthin andβ-apo-8’-carotene acid ethyl ester in feed by ultra performance liquid chromatography

In order to effectively and quickly determine the content of canthaxanthin andβ-apo-8’-carotene acid ethyl ester in feed,the feed was extracted with a mixture of methanol,n-hexane and ethyl acetate,concentrated under nitrogen protection,and the tert-butyl ether-acetonitrile solution was reconstituted and injected into an ultra-high performance liquid chromatograph for determination.The results showed that the concentration of canthaxanthin was in the range of 1.25~10.0μg/mL,and that ofβ-apo-8′-carotene acid ethyl ester was in the range of 2.50~20.0μg/mL had the good linear relationship.The detection limit was 0.06 mg/kg,and the limit of quantification was 0.20 mg/kg.The average recovery rate of 5 additive levels of canthaxanthin was 72.8%~82.8%,and relative standard deviation(RSD)were 0.41%~1.06%;the average recovery of 5 additive levels ofβ-apo-8’-carotene acid ethyl ester was 72.0%~82.8%,and RSD were 0.46%~1.41%.The results indicated that the method was simple,rapid,high sensitivity and good accuracy,and was suitable for the determination of canthaxanthin andβ-apo-8′-carotene acid ethyl ester in feed.