| ELISA检测饲料中莱克多巴胺的不确定度分析 |
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| 引用本文： | 肖静,赵健亚,许世勇,谢怀根,杜方均,刘春.ELISA检测饲料中莱克多巴胺的不确定度分析[J].中国饲料,2012(7):37-39. |
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| 作者姓名： | 肖静 赵健亚 许世勇 谢怀根 杜方均 刘春 |
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| 作者单位： | 南通大学公共卫生学院;南通市动物疫病预防控制中心;南通大学实验动物中心 |
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| 基金项目： | 国家自然科学青年基金资助项目(31000539);南通市科技创新计划(AS2010020);南通市应用研究计划(K2010066) |
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| 摘 要： | 为探讨酶联免疫吸附法(ELISA)检测结果的影响因素,试验分析了ELISA法检测饲料中莱克多巴胺的不确定度。依据JJF 1059-1999《测量不确定度评定与表示》和CNAS-GL06《化学分析中不确定度的评估指南》规定的测量不确定度的基本方法,分析不确定度来源并进行量化,找出主要影响因素。结果显示,ELISA法检测饲料中莱克多巴胺含量为43.75 ng/kg时,其扩展不确定度为6.77 ng/kg,k=2。影响不确定度的主要因素为样品称量、测量重复性和标准曲线拟合。
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| 关 键 词： | 不确定度 酶联免疫法 饲料 莱克多巴胺 |
Uncertainty analysis of ractopamine in feed by ELISA |
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| Institution: | Xiao Jing,Zhao Jianya,Xu Shiyong,et al. |
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| Abstract: | Uncertainty of detection of ractopamine in feed by ELISA was estimated to investigate the effects of each factor on the results.According to the basic method for uncertainty assessment given in JJF 1059-1999 Evaluation and Expression of Uncertainty in Measurement and CNAS-GL06 Guidance on Evaluating the Uncertainty in Chemical Analysis,the sources of uncertainty for determination were analyzed and quantified to find the major affecting factors.The results showed that the content of ractopamine in feed was 43.75 ng/kg and the expanded uncertainty was 6.77 ng/kg(k=2).The major affecting factors of determination were sample weighing,repeatability and standard curve fitting. |
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| Keywords: | uncertainty ELISA feed ractopamine |
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