盐酸川芎嗪体外抗IBDV活性及其作用机制

为在体外成纤维细胞培养体系上探讨盐酸川芎嗪抗鸡传染性法氏囊病病毒的活性及其作用机制，采用MTT法结合细胞病变观察法，通过病毒感染抑制、复制抑制、吸附抑制和直接灭活试验进行评价。结果显示，盐酸川芎嗪在体外对鸡传染性法氏囊病病毒具有较强的作用，其EC50为（92．52±21．13）mg／L，SI≥21．62，最大抑制率达83．7％；盐酸川芎嗪对病毒具有较强的直接杀灭作用，但不能阻止病毒吸附；在复制抑制试验中，盐酸川芎嗪的抗病毒作用具有时间依赖性，分别在病毒吸附O、2、4h时将盐酸川芎嗪加入到体系中，盐酸川芎嗪显示出较强的抗病毒作用，随着病毒吸附时间的延长，盐酸川芎嗪对鸡传染性法氏囊病病毒的抑制作用逐渐减弱。结果表明，盐酸川芎嗪可通过直接杀灭鸡传染性法氏囊病病毒或／和干扰其早期复制过程而表现出抗病毒活性。

Anti-IBDV effect and mechanism of ligustrazine hydrochloride in vitro

Using the method of MTT test and visualization of cytopathologic effect （CPE） assay, ligustrazine hydrochloride（LH） was tested for its antiviral activity on infectious bursal disease virus （IBDV） in chick embryo fibroblast （CEF） by antiviral assay, time-of-addition assay,adsorption inhibition assay and virucidal assay. Results showed that LH could reduce the damage of IBDV to CEF, with the inhibition ratio exceeding to 83.70;, at the maximum no-cytotoxic concentration. The value of EC50 is （92.52±21.13） mg/L and SI≥21.62. LH had strong anti-IBDV effect in virucidal assay, but have no effect in adsorption inhibition assay. Time-of-addition assay showed that LH exhibited a strong anti-IBDV activity when LH was added at （0 h） or after （2 h and 4 h） infection,and the anti-IBDV effect of LH was decline with the increasing infectious time （from 6 h to 16 h）. The results suggested that LH antiviral activity is attributed to directly inactivate and/ or disturb the early stage of IBDV replication.