E．stiedai感染兔免疫器官内球虫抗原的免疫组化定位

用免疫组化SABC法，对斯氏艾关耳球虫感染后兔肝脏、脾脏和肝门淋巴结中球虫抗原的分布进行了观察。结果表明，在肝脏窦状隙、胆管上皮细胞、肝门淋巴结和脾脏内可见到明显的呈棕黄色阳性反应区，表明在这些区域和部位有相应的E．stiedai孢子囊、裂殖子或子孢子抗原存在，且抗原数量以肝脏内最多，其次为脾脏，最少者为肝门淋巴结；显微镜下还可见到大量坏死的肝细胞、淋巴细胞以及朗罕氏巨细胞，说明多核巨细胞也参与了对球虫的吞噬和处理过程。

Immunohistochemical localization of E. stiedai antigen in immune organs of rab- bits during infection

The distribution of E. stiedai antigen in the liver,spleen and lymph nodes from the hepa toduodenal ligament of rabbits was determined by immunohistochemical SABC method. The re sults suggested that E. stiedai antigen, such as sporocysts, schizozoites and sporozoites were dis tributed in hepatic sinusoid, bile duct epithelial cells,portal lymph node and spleen,according to the obvious brownyellow positive reaction zone. The quantity of antigen in order was liver, spleen and portal lymph node. There were also lots of necrotic liver cells,lymphocytes and Langhan＇s gi ant cells in the immune organs. It indicated that multiple nuclear giant cells were involved in the process of phagocytosis and treatment on coccidia.