牛MSTN基因克隆及编码区E1-224-A〉C定点突变前后生物信息学对比分析

牛肌肉生长抑制素（Myostatin,MSTN）主要是骨骼肌生长发育的负调节因子,能够限制肌纤维的增粗增大,其前两个外显子共同编码N-端前肽,第三外显子编码C-端多肽。突变其前肽编码序列会阻止牛MSTN基因表达蛋白与相应受体的结合,导致MSTN功能失活,促进肌肉增值,但具体突变位点仍未确定。本研究设计特定的引物对牛MSTN基因编码区进行PCR扩增并进行了克隆及测序,并假定牛MSTN基因编码序列发生E1-224-A〉C点突变,通过生物信息学的方法,对牛MSTN基因突变前后编码产物的理化性质、结构与功能进行了对比分析,为MSTN基因结构与功能的研究奠定了基础。

Cloning of bovine MSTN gene and bioinformatics comparative analysis between- coding region with E1-224-A〉C mutation site and normal type.

The growth of muscle fibers can be negatively regulated by the bovine myostatin gene. The first two exons of this gene code for the N-propeptide,while the third exon codes for the C- polypeptide. The structure of the leader peptide is altered via mutation to combine more closely with the mature peptide and inhibit MSTN gene functioning. However,the specific mutations are uncertain. In this study, the coding region of the bovine MSTN gene was amplified via specific primers by PCR method, and the sequence was cloned and sequenced. Bioinformatics methods were taken for a comparative analysis of physical and chemical properties, structure and function between mutant type and normal type, assuming that bovine MSTN gene coding sequence with E1-224-A〉C mutation site. The research lays a foundation for the studies on the structure and function of bovine MSTN gene.