钼对TM3小鼠睾丸间质细胞周期和凋亡的影响

以体外培养的小鼠睾丸间质细胞系TM3 为材料,加入不同质量浓度的钼酸钠溶液（0,10,20,40,80,160mg/L）染毒培养,分别在干预4,8,12,24,48h采用MTT法检测细胞的增殖。干预48h后,采用流式细胞术检测细胞周期和凋亡,单细胞凝胶电泳检测DNA损伤的变化。结果表明：与对照组相比,不同剂量钼酸钠作用24h后,睾丸间质细胞的增殖活性均受到抑制;不同质量浓度的钼酸钠作用48h后,细胞周期阻滞于G0/G1期,20mg/L及其以上剂量组G0/G1期细胞百分率与对照组相比显著升高（P〈0.05或P〈0.01）;与对照组相比,各剂量组TM3 小鼠睾丸间质细胞凋亡率显著升高,差异极显著（P〈0.01）;细胞尾部DNA含量及细胞尾长随着钼剂量的增加呈不同程度的增加,且存在着剂量—效应关系。结论说明钼能够引起睾丸间质细胞周期的紊乱,并诱导睾丸间质细胞发生DNA损伤和凋亡。

Effect of molybdenum on the cell cycle and apoptosis of TM3 mouse Leydig cells

The aim of the study was to evaluate the effects of sodium molybdate on cell cycle,apop- tosis and DNA damage of TM3 mouse Leydig cells in vitro. TM3 mouse Leydig cells were exposed to different concentrations of sodium molybdate （0,10,20,40,80 and 160 mg/L） for 48 h. Each group was treated for 4,8,12,24 and 48 h for MTT test. Flow cytometry and single cell gel elec- trophoresis were applied to detect the cell cycle,apoptosis and DNA damage of TM3 mouse Leydig cells in each group after treated for 48 h. The results showed that compared with the control group （0 mg/L）,the proliferation of the Leydig cells were inhibited after treated by sodium molybdate for 48h. As tested by FCM,TM3 mouse Leydig cells in G0/G1 phase were much higher （P〈0.05 and P〈0.01） at≥20 mg/L sodium molybdate than in control group. Compared with the control group,the percentage of apoptosis was significantly increased （P〈0.01）. The tail length and tail DNA % were increased with the increasing of molybdenum in dose-dependent when compared with that of control. The results showed that molybdenum could cause cell cycle arrest, and induce ap- optosis and DNA damage of Leydig cells.