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吉林省2006--2010年羊传染性脓疱病的流行病学调查及分析
引用本文:郭良兴,赵魁,赵广海,贺文琦,袁洁,陆慧君,王改丽,苏高莉,高丰.吉林省2006--2010年羊传染性脓疱病的流行病学调查及分析[J].兽医大学学报,2011(11):1623-1626.
作者姓名:郭良兴  赵魁  赵广海  贺文琦  袁洁  陆慧君  王改丽  苏高莉  高丰
作者单位:[1]吉林大学畜牧兽医学院,吉林长春130062 [2]郑州牧业工程高等专科学校,河南郑州450045 [3]辽宁省本溪满族自治县动物疫病预防控制中心,辽宁本溪117100 [4]广东省农业科学院兽医研究所,广东广州510640 [5]吉林大学人兽共患病研究所,吉林长春130062
基金项目:国家自然科学基金资助项目(31101785);吉林省科技厅重点计划发展项目(20080217);吉林大学基本科研业务费平台基地建设项目(450060326051)
摘    要:为了解近年来羊传染性脓疱病在吉林省的流行情况,本试验采用PCR方法对2006—2010年间采自吉林省9个不同市县85个养羊户疑似羊传染性脓疱病毒(ORFV)感染的628份痂皮样本(背部、乳房、四肢以及尾根等部位皮肤)进行羊传染性脓疱病毒核酸的检测,在各市县不同年龄不同品种羊的皮肤痂皮样本中均检测到一定程度的ORFV,平均阳性率为24.36%(153/628),其中以1~6月龄羔羊的阳性检出率最高。应用间接ELISA方法对同期采集自上述地区羊群中的2160份血清样本进行ORFV抗体检测,其中有852份呈现0RFV抗体阳性反应,平均阳性率为39.44%(852/2160)。以上结果表明,ORFV在吉林省大部分地区羊群中的感染已经非常普遍。

关 键 词:羊传染性脓疱病  PCR  ELISA  流行病学

Epidemiological investigation and analysis of contagious ecthyma in Jilin province from 2006 to 2010
Authors:GUO Liang-xing  ZHAO Kui  ZHAO Guang-hai  HE Wen-qi  YUAN Jie  LU Hui-j un  WANG Gai-li  SU Gao-li  GAO Feng
Institution:1. College of Animal Science and Veterinary Medicine, J ilin University, Changchun 130062, China ;2. Zhengzhou Animal Husbandry Engineering Institute, Zhengzhou 450045,China;3. Animal Diseases Prevention and Control Center of Benxi Man Peo- ple Autonomy County, Benxi, Liaoning 117100, China ; 4. Institute of Guangdong Veterinary Sci ence ,Guangzhou 510640, China; 5. Institute of Zoonosis , J ilin University, Changchun 130062, China)
Abstract:In order to understand the recent epidemiology knowledge of contagious ecthyma in J ilin province,628 scab samples (including the skins of the back,udder,limbs and tail) collected from 85 flocks in 9 different areas of Jilin province from 2006 to 2010 were detected by PCR. The viral nucleic acid of Orf virus could be detected from scab samples,the average positive rate was 24.36% (153/628) ,and the positive rate reached the highest in lambs aged 1- 6 months. The ORFV antibodies of 2 160 serum samples collected from the flocks mentioned above were detected by indirect ELISA. The result showed that 852 serum samples were positive,and the average positive rate was 39.44% (852/2 160) . The result indicated that ORFV infection was very widespread in the flocks of Jilin province.
Keywords:contagious ecthyma  PCR  ELISA  epidemiology
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