| 卵泡抑素对牛胚胎早期发育的影响 |
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| 引用本文： | 郭镇华,刘娣,马红,李忠秋,付博,张东杰,汪亮,冯艳忠,唐晓东,张淼,王欣,张海波.卵泡抑素对牛胚胎早期发育的影响[J].兽医大学学报,2012(9):1299-1301,1304. |
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| 作者姓名： | 郭镇华 刘娣 马红 李忠秋 付博 张东杰 汪亮 冯艳忠 唐晓东 张淼 王欣 张海波 |
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| 作者单位： | 黑龙江省农业科学院,黑龙江哈尔滨150086 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助项目（CARS-36）; 转基因生物新品种培育重大专项（2008ZX08006-003）; 国家自然科学基金资助项目（31072020） |
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| 摘 要： | 利用添加外源性卵泡抑素或抑制卵泡抑素基因表达的方法,以牛受精胚胎为研究对象,结果显示,胚胎阶段适当的添加卵泡抑素,可以加快胚胎发育,不但提高卵裂率,还可以在受精后7d提高囊胚发育率,这其中10μg/L的卵泡抑素为最佳添加量,达到35%的囊胚率。研究还发现,早期阶段沉默卵泡抑素基因,不会影响囊胚率,与处理组无显著差别。结果表明,可能在早期的胚胎发育的最初阶段,卵泡抑素并不是必须的,但如果外源性的添加适量的卵泡抑素可以在正常的基础上提高发育率。
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| 关 键 词： | 牛 卵泡抑素 胚胎 |
Follistatin affects early development of bovine embryos |
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| Authors: | GUO Zhen-hua LIU Di MA Hong LI Zhong-qiu FU Bo ZHANG Dong-jie WANG Liang FENG Yan-zhong TANG Xiao-dong ZHANG Miao WANG Xin ZHANG Hai-bo |
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| Institution: | (Heilongjiang Academy Agriculture of Science, Harbin 150086 ,China) |
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| Abstract: | Use exogenesis follistatin and silence FST gene to affect the development of bovine early embryo. The results shows that in the embryonic stage, adding follistatin, can accelerate development, not only increase cleavage rate, but also improve the blastocyst rates at 7 days after fertilization, which 35% of the blastocyst rates was observed at 10 μg/L of follistatin. In early stage, silent FST gene will not affect the blastocyst rate. The result showed that in the initial stages of early embryonic development, follistatin is not necessary,however adding exogenesis follistatin can accelerate its development. |
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| Keywords: | bovine follistatin embryos |
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