| 不同供体细胞代数对兔体细胞核移植效率的影响 |
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| 引用本文： | 宋伟杰,谷瑞环,尹明茹,李敦高,邢凤英.不同供体细胞代数对兔体细胞核移植效率的影响[J].兽医大学学报,2012(7):1060-1064,1072. |
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| 作者姓名： | 宋伟杰 谷瑞环 尹明茹 李敦高 邢凤英 |
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| 作者单位： | [1]东北林业大学野生动物资源学院,黑龙江哈尔滨150040 [2]上海交通大学实验动物科学部,上海200025 |
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| 基金项目： | 上海市科委科研计划项目（09140901000,10140901700）;上海市科委自然科学基金资助项目（11ZR1418800） |
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| 摘 要： | 将兔成纤维细胞分为5～10低代数组和20～25高代数组进行核移植研究。结果显示，2试验组供体细胞核移植的效率存在差异。通过对供体细胞进行核移植后的重构胚胎的融合率、卵裂率、囊胚率以及体内发育的比较，发现低代数相对来说更有效率，但是高代数组也能获得正常的克隆后代。结果表明，兔不同代数体外培养细胞对克隆效率存在影响，并为制备转基因打靶兔提供基础。
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| 关 键 词： | 兔 成纤维细胞 体细胞核移植 细胞代数 基因打靶兔 |
Effect of different passages of donor cell on the efficiency of rabbit somatic nuclear transfer |
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| Authors: | SONG Wei-jie GU Rui-huan YIN Ming-ru LI Du Xue-jin LI He-ping College of Wildlife bin China ; Laboratory Animal Centre S China n-gao XING Feng-ying |
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| Institution: | z , LI Shan-gangz , CHEN Resource ,Northeast Forestry University , Har- hanghai J iaotong University, Shanghai 200025, |
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| Abstract: | The time and cultivate passage of donor cells in vitro culturing has a significant effect on the efficiency of somatic cloning. We carry on a research to 5-10 low generation group and 20-25 high generation group of rabbit fi- broblasts by nuclear transfer,and found that the efficiency of transfered donor nuclei in two experimental groups are differents. Through comparison of the fusion rate,cleavage rate,blastocysts rate and in vivo development of the re- constructed embryos after donor cell nuclear transfering, relatively low generation was found more efficient, but high generation can also get normal cloned offspring. This study verifys the influence of different passages cells of in vitro culturing on the efficiency of rabbit cloning,and provides basis for cloning gene targeting rabbits. |
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| Keywords: | rabbit adult fibroblast somatic cell nuclear transfer cell passage gene targeting rabbit |
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