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| 基于多重PCR的寡核苷酸基因芯片检测猪瘟病毒,猪繁殖与呼吸障碍综合征病毒及猪圆环病毒1型和2型 | |
| 引用本文： | 姜永厚,商晗武,徐辉,朱良俊,丁先锋,陈伟杰,赵灵燕,方立.基于多重PCR的寡核苷酸基因芯片检测猪瘟病毒,猪繁殖与呼吸障碍综合征病毒及猪圆环病毒1型和2型[J].中国预防兽医学报,2010,32(3). |
| 作者姓名： | 姜永厚 商晗武 徐辉 朱良俊 丁先锋 陈伟杰 赵灵燕 方立 |
| 作者单位： | 1. 浙江理工大学生命科学学院,浙江,杭州310018 2. 中国计量学院生命科学学院,浙江,杭州310018 3. 浙江省动物疫病预防控制中心,浙江,杭州310020 4. 浙江大学动物科学学院,浙江,杭州310029 |
| 基金项目： | 浙江省科技计划项目，浙江省自然科学基金项目，浙江省科技攻关重点项目 |
| 摘 要： | 为了建立一种可同时检测区分猪瘟病毒(CSFV),猪繁殖与呼吸障碍综合征病毒(PRRSV)和猪圆环病毒2型(PCV-2)的寡核苷酸基因芯片方法,本研究针对每种病毒设计了4条～6条寡核苷酸探针,检测低限为1.6×104PCV-2拷贝数/μL,1.6×104CSFV拷贝数/μL,1.6×105PRRSV拷贝数/μL,比琼脂糖凝胶灵敏约10倍。利用建立的寡核苷酸基因芯片方法对76个仔猪样本进行了检测,检出了3种病毒的存在,其中25个样本(32.9%)同时感染了2种以上病毒。结果表明寡核苷酸基因芯片检测是一种快速、灵敏和高效的猪只混合感染病毒的病原学诊断方法。 |
| 关 键 词： | 基因芯片 猪瘟病毒 猪繁殖与呼吸障碍综合征病毒 猪圆环病毒 |
A multiplex PCR based oligonucleotide microarray for detection and differentiation of classical swine fever virus,porcine reproductive and respiratory virus and porcine circovirus types 1 and 2 |
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| JIANG Yong-hou,SHANG Han-wu,XU Hui,ZHU Liang-jun,DING Xian-feng,CHEN Wei-jie,ZHAO Ling-yan,FANG Li.A multiplex PCR based oligonucleotide microarray for detection and differentiation of classical swine fever virus,porcine reproductive and respiratory virus and porcine circovirus types 1 and 2[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). | |
| Authors: | JIANG Yong-hou SHANG Han-wu XU Hui ZHU Liang-jun DING Xian-feng CHEN Wei-jie ZHAO Ling-yan FANG Li |
| Institution: | JIANG Yong-hou1,SHANG Han-wu2,XU Hui3,ZHU Liang-jun1,DING Xian-feng1,CHEN Wei-jie3,ZHAO Ling-yan3,FANG Li4 (1. College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China,2. College of Life Sciences,China Jiliang University,3. Centre of Animal Diesase Control of Zhejiang Province,Hangzhou 310020,4. College of Animal Sciences,Zhejiang University,Hangzhou 310029,China) |
| Abstract: | Porcine circovirus type 2 (PCV-2),classical swine fever virus (CSFV) and porcine reproductive and respiratory syn-drome virus (PRRSV) are important viruses that cause severe reproductive and/or respiratory failure in swine,and rapid and accu-rate detection and identification of these pathogens is critical for swine disease management. In the present study,we developed a multiplex polymerase chain reaction (PCR) based oligonucleotide microarray assay that included four to six specific oligonu-cleotides for e... |
| Keywords: | Microarray Detection Classical swine fever virus Porcine reproductive and respiratory virus Porcine cir-covirus types 1 and 2 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |