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| 表达犬瘟热病毒H基因重组新城疫病毒的构建及其免疫原性研究 | |
| 引用本文： | 田美杰,葛金英,王喜军,冯娜,马良,帅磊,苏华,高玉伟,夏咸柱,步志高.表达犬瘟热病毒H基因重组新城疫病毒的构建及其免疫原性研究[J].中国预防兽医学报,2012,34(4):257-261. |
| 作者姓名： | 田美杰 葛金英 王喜军 冯娜 马良 帅磊 苏华 高玉伟 夏咸柱 步志高 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部兽医公共卫生重点开放实验室,黑龙江哈尔滨,150001 2. 军事医学科学院军事兽医研究所,吉林长春,130062 3. 东北农业大学动物医学学院,黑龙江哈尔滨,150030 |
| 基金项目： | 国家自然科学基金青年科学基金项目(30800816) |
| 摘 要： | 为研制安全、有效的新型犬瘟热疫苗,本研究利用新城疫病毒(NDV) LaSota弱毒疫苗株反向遗传操作系统,构建出表达犬瘟热病毒(CDV)弱毒疫苗株Rockborn-20/8血凝素(H)蛋白的重组病毒rLa-CDV-H,并对其生物学特性进行鉴定,评估其作为犬瘟热活载体疫苗的安全性和有效性.通过免疫荧光和western blot试验证明了CDV H蛋白的正确表达；重组病毒株保持了LaSota亲本株的低致病性和高滴度鸡胚生长特性；重组病毒rLa-CDV-H接种12周龄比格犬后,可以诱导显著的CDV中和抗体反应.本研究表明重组病毒rLa-CDV-H具有作为犬瘟热重组病毒活载体疫苗的潜力. |
| 关 键 词： | 犬瘟热病毒 血凝素蛋白 重组新城疫病毒 |
Construction of Newcastle disease virus-vectored canine distemper virus(CDV) vaccine and induction of CDV neutralizing antibody |
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| TIAN Mei-jie , GE Jin-ying , WANG Xi-jun , FENG Na , MA Liang , SHUAI Lei , SU Hua , GAO Yu-wei , XIA Xian-zhu , BU Zhi-gao.Construction of Newcastle disease virus-vectored canine distemper virus(CDV) vaccine and induction of CDV neutralizing antibody[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):257-261. | |
| Authors: | TIAN Mei-jie GE Jin-ying WANG Xi-jun FENG Na MA Liang SHUAI Lei SU Hua GAO Yu-wei XIA Xian-zhu BU Zhi-gao |
| Institution: | 1(1.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Insitute,Chinese Academy of Agricultural Sciences, Harbin 150001,China;2.The Military Veterinary Institute,Academy of Military Sciences,Changchun 130062,China; 3.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: | In order to develop a safe and efficient canine distemper vaccine based on Newcastle disease virus(NDV) La Sota vaccine strain,we constructed and rescued a recombinant NDV which expressed hemagglutinin(H) protein of canine distemper virus(CDV,Rockborn-20/8 strain),named rLa-CDV-H,by reverse genetics techniques.The expression of CDV H protein in recombinant virus was confirmed by western blot and indirect immunofluorescence assay.rLa-CDV-H retained its high-titer growth property and low pathogenicity in embryonated chicken eggs.Moreover,rLa-CDV-H induced significant CDV neutralizing antibodies in 12 week old dogs post-inoculation.This result indicated that rLa-CDV-H was able to induce efficiently immune responses in dogs,which showed that rLa-CDV-H had potential of being a novel CDV vaccine. |
| Keywords: | canine distemper virus hemagglutinin protein recombinant NDV |
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