| 传染性法氏囊病RT-PCR诊断方法研究 |
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| 引用本文： | 荣俊,刘晓娜,程太平,杨待建.传染性法氏囊病RT-PCR诊断方法研究[J].中国预防兽医学报,2000(Z1). |
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| 作者姓名： | 荣俊 刘晓娜 程太平 杨待建 |
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| 作者单位： | 湖北农学院动物科学系!荆州 434103 |
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| 摘 要： | 本文在研究传染性法氏囊病病毒的Ａ片断ｃＤＮＡ结构的基础上，用Ｐｒｉｍｅｒ Ｄｅｓｉｇｎ引物设计软件，在ＶＰ_５和ＶＰ_２的重叠基因区设计了一对引物，使用该引物对６种ＩＢＤＶ的标准毒株，１０例自然发病病鸡组织标本，８例ＩＢＤＶ Ｊ_１Ｃ_７Ｆ_３种毒株攻毒病理法氏囊组织标本，进行了ＲＴ－ＰＣＲ检测均得到了阳性的扩增结果。２种参考鸡病病毒和８例健康鸡的法氏囊组织在同样条件下进行扩增均为阴性结果。本法具有扩增方法简单，对各种病毒株的适应性广，特异性高，检测成本较低的特点。
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| 关 键 词： | IBDV RT-PCR 诊断试剂 |
Diagnosis of IBDV with RT-PCR |
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| RONG Jun,LIU Xiaona,CHENG Taiping,YANG Daijian.Diagnosis of IBDV with RT-PCR[J].Chinese Journal of Preventive Veterinary Medicine,2000(Z1). |
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| Authors: | RONG Jun LIU Xiaona CHENG Taiping YANG Daijian |
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| Abstract: | After a carefully study on segment A of the cDNA of infectious bursal disease virus(IBDV), a pair of primers were designed with the Primer Design software.This primer pair is in the overlapping area of VP5 and VP2 where the sequence is highly homologous.6 stan- dard virus strains, 10 tissue specimen of natural diseased chickens and 8 pathology cloacial bursa specimen of IBDV J1C7F3 attacking test chickens had been amplified successfully with this pair of primers. 2 other comparison virus strains and 8 healthy chickens' cloacial bursa had given negative results. The system has the following features: it has wide spread adjustability to various kinds of virus; the specificity is high; the process is ease to oerate; and the test cost is low. |
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| Keywords: | IBDV RT-PCR Diagnosis reagent |
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