鸡致病性大肠杆菌1型菌毛fimI基因克隆与序列测定

对鸡O2血清型致病性大肠杆菌1型菌毛fimI基因进行了扩增并与国外同源菌株基因序列进行了比较。结果表明：两者核苷酸同源性达98．42％，预测氨基酸顺序同源性达98．92％。fimI基因的预测氨基酸序列中仅第46位氨基酸由ALA变为THR，第76位氨基酸由SER变为ALA，其余核苷酸的变化未影响氨基酸的翻译，推测，这种改变是由分离株的差异所引起的。

Clonging and sequencing of fimI gene from avian pathogenic E.coH

The nueleotide sequence of fimI gene from avian pathogenic E.coli O2 isolate was determined. The homology of nucleotide sequences of fimI gene was 98.42 %, in comparison with the data in Genhank, and the homology of amino acid sequences of FimI proteins encoded by fimI was 98.92 %. There were only two sites changed in the amino acid sequences. One was THR in the forty-sixth site, instead of ALA. The other was ALA in the seventy-sixth site, instead of SER. It suggested that the changes were due to the diversity of isolates.