| 不同H5N1亚型禽流感病毒在豚鼠体内的复制和传播能力评估 |
| |
| 引用本文： | 张莹,高玉伟,邓国华,姜永萍,樊树芳,钟功勋,关云涛,陈化兰.不同H5N1亚型禽流感病毒在豚鼠体内的复制和传播能力评估[J].中国预防兽医学报,2010,32(3). |
| |
| 作者姓名： | 张莹 高玉伟 邓国华 姜永萍 樊树芳 钟功勋 关云涛 陈化兰 |
| |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所国家禽流感参考实验室,黑龙江,哈尔滨150001
2. 中国农业科学院哈尔滨兽医研究所国家禽流感参考实验室,黑龙江,哈尔滨150001;军事医学科学院第11研究所,吉林,长春130062 |
| |
| 摘 要： | 本研究以豚鼠作为哺乳动物模型,评价了6株H5N1亚型禽流感病毒(AIV)的复制和水平传播能力。经滴鼻接种后,检测到6株病毒中有5株病毒在豚鼠上呼吸道和下呼吸道复制,病毒滴度为0.8LogEID50/mL～3.5LogEID50/mL(或g),豚鼠在感染后第2d至第10d可排出病毒。另外一株病毒A/duck/GX/22/02则仅能在豚鼠的下呼吸道低水平复制。在传播实验中,6株病毒中只有A/duck/GX/35/01能够在豚鼠间水平传播。上述研究结果表明,豚鼠适用于作为高致病性AIV哺乳动物水平传播的模型,而且H5N1亚型AIV在哺乳动物间的水平传播能力不同。本研究为进一步研究AIV在哺乳动物间水平传播的分子机制奠定了良好的基础。
|
| 关 键 词： | 禽流感病毒 H5N1亚型 豚鼠 复制 传播 |
Evaluation of replication and transmissibility of different H5N1 avian influenza virus in guinea pigs |
| |
| ZHANG Ying,GAO Yu-wei,DENG Guo-hua,JIANG Yong-ping,FAN Shu-fang,ZHONG Gong-xun,GUAN Yun-tao,CHEN Hua-lan.Evaluation of replication and transmissibility of different H5N1 avian influenza virus in guinea pigs[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). |
| |
| Authors: | ZHANG Ying GAO Yu-wei DENG Guo-hua JIANG Yong-ping FAN Shu-fang ZHONG Gong-xun GUAN Yun-tao CHEN Hua-lan |
| |
| Institution: | ZHANG Ying1,GAO Yu-wei1,2,DENG Guo-hua1,JIANG Yong-ping1,FAN Shu-fang1,ZHONG Gong-xun1,GUAN Yun-tao1,CHEN Hua-lan1 (1. National Avian Influenza Reference Lab,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China,2. Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130062,China) |
| |
| Abstract: | In this study,the replication and transmissibility of 6 H5N1 avian influenza virus strains was evaluated using guinea pig as a mammalian model. Five of the 6 virus strains could be detected in both the upper and lower respiratory tract of guinea pigs from 2 to 10 day after virus infection. However,the A/duck/GX/22/02 strain could only replicate in the lower respiratory tract. Among tested virus strains,only A/duck/GX/35/01 virus was shown to transmit from guinea pig-to-guinea pig. Our results indicated that... |
| |
| Keywords: | avian influenza H5N1 subtype guinea pig transmission animal model |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
