| 4株IBV中国地方分离株部分结构基因变异的研究 |
| |
| 引用本文： | 任涛,廖明,曹伟胜,辛朝安.4株IBV中国地方分离株部分结构基因变异的研究[J].中国预防兽医学报,2006,28(3):285-288. |
| |
| 作者姓名： | 任涛 廖明 曹伟胜 辛朝安 |
| |
| 作者单位： | 华南农业大学,兽医学院禽病学教研室,广东,广州,510640;农业部,养禽与禽病防治重点开放实验室,广东,广州,510640 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);广东省教育厅SARS专项基金 |
| |
| 摘 要： | 本研究通过RT-PCR分别获得了4个国内IBV分离株的S1、M和N基因，并进行了克隆及测序。序列分析结果表明：HaN2-95株的S1、M和N基因核苷酸序列均与IBV H120疫苗株的同源性最高；尽管HaN1-95株的S1基因与IBV H52疫苗株的亲缘关系最近，但是该毒株的M基因和N基因却与IBV Gray株的同源性最高；GX1-98株的S1和M基因均与IBV H52疫苗株的亲缘关系最近，但其N基因却与IBV Gray株和Ark99株有高度的同源性；GX2-98株的S1基因却与IBV Holte株的亲缘关系最近。上述结果提示国内有些IBV分离株的出现可能与疫苗株的使用有关。
|
| 关 键 词： | 结构基因 变异 |
| 文章编号： | 1008-0589(2006)03-0285-04 |
| 收稿时间： | 2005-02-26 |
| 修稿时间： | 2005年2月26日 |
Study on variation of parts of structural genes of 4 infectious bronchitis virus isolates from China |
| |
| REN Tao,LIAO Ming,CAO Wei-sheng,XIN Chao-an.Study on variation of parts of structural genes of 4 infectious bronchitis virus isolates from China[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):285-288. |
| |
| Authors: | REN Tao LIAO Ming CAO Wei-sheng XIN Chao-an |
| |
| Institution: | 1. College of Veterinary Medicine,South China Agricultural University, Guangzhou 510640, China ; 2. The Agricultural Ministry Key Laboratory of Poultry Feeding and Disease Control, Guangzhou 510640, China |
| |
| Abstract: | S1,M,N gene fragments of four Chinese local infectious bronchitis virus isolates were obtained by RT-PCR in the study,then cloned and sequenced respectively.Sequences analysis showed: S1,M,N gene of strain HaN2-95 all had the highest similarities with that of IBV H120 vaccine strain;S1 gene of strain HaN1-95 had the closest genetic relationship with IBV H52 vaccine strain,while the M,N gene of strain HaN1-95 had the highest similarities with that of strain Gray;although the S1 and M gene of strain GX1-98 all had the highest genetic relationship with that of IBV H52 vaccine strain,yet the N gene of strain GX1-98 had high similarity with that of IBV Ark99 strain or Gray strain;S1 gene of GX2-98 strain showed the high similarities with that of IBV Holte strain.The above results suggested that emergence of some Chinese local IBV strain seems to be related with broad usage of IBV live vaccine strains. |
| |
| Keywords: | IBV |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
