不同方法对鸡胚和法氏囊中囊病病毒抗原含量的检测比较

用单抗介导的抗原捕获－酶联免疫吸附试验（AC－ELISA），琼脂扩散试验（ACP）快速诊断试纸条（ISK）对感染同株强毒或弱毒囊病病毒（IBDV）的鸡胚和鸡法氏囊中的病毒抗原效价进行了检测比较，结果表明，上述3种方法测得法氏囊中温毒IBDV抗原效价分别为10^6.0(AC-ELISA),2^-3.0(AGP)t 10^-3.0，测得鸡胚中强毒IBDV抗原效价依次为10^-1.0,0和0。强毒株接种鸡胚后，只有第1代鸡胚组织可被AC－ELISA测到低效价的IBDV抗原，而第2代以后则未能测到病毒抗原，弱毒疫苗株IBDV感染鸡后，法氏囊中可测到低效价抗原，但感染鸡胚后则无可测性病毒抗原。

Comparison of IBDV Antigen Titers between Bursa and Embryo Detected by Different Methods

Antigen_capture enzyme_linked immlnoadsorption assay (AC_ELISA) mediated by monoclonal antibodies,agar gel precipitation(AGP) and fast indicator slip kit (FIS) were used for detection of infectious bursal disease virus (IBDV) antigen in both SPF and commercial chicken bursas and embryos inoculated with virulent and attenuated strains.The antigen titers of the virulent samples detected by AC_ELISA, AGP and FIS were 10 -6.0 , 2 -3.0 ,and 10 -3.0 in bursas, separately, and 10 -1.0 , O and O in embryos, separately. The virulent IBDV antigen was detected only by AC_ELISA from the first passage embryos only, and was not detected by all three methods from the subseqent passages. Low antigen titer of attenuated IBDV was only detected from infected bursa by AC_ELISA but not from embryo by all three methods.