| 传染性喉气管炎病毒王岗株糖蛋白gB基因在重组杆状病毒中的表达 |
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| 引用本文： | 张绍杰,倪健强,孟松树,王柳,仇华吉,王云峰,童光志.传染性喉气管炎病毒王岗株糖蛋白gB基因在重组杆状病毒中的表达[J].中国预防兽医学报,2001,23(5):321-324. |
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| 作者姓名： | 张绍杰 倪健强 孟松树 王柳 仇华吉 王云峰 童光志 |
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| 作者单位： | 哈尔滨兽医研究所中国农业科学院 |
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| 摘 要： | 将克隆到pUC119中的传染性喉气管炎病毒（ILTV）糖蛋白gB基因，通过EcoRI位点克隆至杆状态病毒转移载体pVL1393中，构建成重组杆状病毒转移载体rpVLgB，将rpVLgB转移载体质粒与杆状态病毒DNA（Bac-N-Blue DNA)共转染Sf9昆虫细胞，经3轮蚀斑纯化，获得重组病毒并命名为rpVL－ILTVgB。PCR方法鉴定证明gB基因正确插入到杆状病毒基因组中，直接免疫荧光试验和Dot－ELISA结果均表明gB基因在重组杆状病毒感染的Sf9昆虫细胞保获得表达，表达的gB蛋白将作为鸡传染性喉气管炎的亚单位疫苗和诊断抗原。
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| 关 键 词： | 传染性喉气管炎病毒 糖蛋白gB 重组杆状病毒 基因 鸡病毒 |
| 文章编号： | 1008-0589(2001)05-0321-04 |
| 修稿时间： | 2000年10月30 |
Expression of Glycoprotein B(gB)of Infectious Laryngotracheitis Virus Strain Wanggang in Insect Cells Infected with a Recombinant Baculovirus |
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| ZHANG Shao_jie,NI Jian_qiang,MENG Song_shu,WANG Liu,QIU Hua_ji,WANG Yun_feng,TONG Guang_zhi.Expression of Glycoprotein B(gB)of Infectious Laryngotracheitis Virus Strain Wanggang in Insect Cells Infected with a Recombinant Baculovirus[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(5):321-324. |
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| Authors: | ZHANG Shao_jie NI Jian_qiang MENG Song_shu WANG Liu QIU Hua_ji WANG Yun_feng TONG Guang_zhi |
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| Institution: | ZHANG Shao_jie,NI Jian_qiang,MENG Song_shu,WANG Liu,QIU Hua_ji,WANG Yun_feng,TONG Guang_zhi* |
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| Abstract: | The gB gene of infectious laryngotracheitis virus(ILTV)strain WG was subcloned into the Baculovirus transfer vector pVL1393 and a recombinant vector rpVLgB was generated. Co_transfection of Sf9 insect cells with rpVLgB and Baculovirus linear DNA was performed (BAC_N_Blue DNA).After three times purification,a recombinant baculovirus designated as rpVL_ILTVgB was obtained. Expression of ILTV gB in rpVL_ILTVgB infected Sf9 cells was detected by direct immunofluorescence and Dot_ELISA.The expressed glycoprotein B will be used as subunit vaccine or diagnostic antigen. |
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| Keywords: | ILTV Glycoprotein B Recombinant baculovirus |
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