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| 猪戊型肝炎病毒大庆株DQ1全基因序列分析 | |
| 引用本文： | 于敏,曲娟娟,郭巍,赵立平,相文华.猪戊型肝炎病毒大庆株DQ1全基因序列分析[J].中国预防兽医学报,2006,28(5):507-510. |
| 作者姓名： | 于敏 曲娟娟 郭巍 赵立平 相文华 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001;东北农业大学,黑龙江,哈尔滨,150030 2. 东北农业大学,黑龙江,哈尔滨,150030 3. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 |
| 摘 要： | 本试验采用RT．-PCR的方法对戊型肝炎病毒大庆株（DQ1HEV株）的全基因进行分片段扩增，并对其两个末端采用末端快速扩增法（RACE）进行扩增、克隆，测序。与已报道的人的14株HEV的4个基因型的核苷酸和氨基酸进行比较，与IV型HEV的同源性最高。ORF1区与IV型HEV核苷酸的同源性为82．6％～83．6％．氨基酸同源性为93．5％，ORF2区与IV型HEV核苷酸的同源性为87．0％～88.4％，氨基酸同源性为95．8％～97．4％。ORF3区与IV型HEV核苷酸的同源性为94．4％～96．5％，氨基酸同源性为90.3％～96．5％。其结果表明DQ1 HEV株为IV型HEv。 |
| 关 键 词： | 猪戊型肝炎病毒 克隆 序列分析 |
| 文章编号： | 1008-0589(2006)05-0507-04 |
| 收稿时间： | 2005-07-12 |
| 修稿时间： | 2005年7月12日 |
The gene cloning and sequence analysis of the complete genome of swine Hepatitis E virus DQ1 strain |
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| YU Min,QU Juan-juan,GUO Wei,ZHAO Li-Ping,XIANG Wen-Hua.The gene cloning and sequence analysis of the complete genome of swine Hepatitis E virus DQ1 strain[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(5):507-510. | |
| Authors: | YU Min QU Juan-juan GUO Wei ZHAO Li-Ping XIANG Wen-Hua |
| Institution: | 1. Division of Livestock Infectious Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 15001, China; 2. Northeast Agricultural University, Harbin 150030, China |
| Abstract: | One HEV isolate from a swine in Daqing were sequenced over the entire genome by RT-nested PCR and the 5' and 3'ends were amplified with RACE,and compared phylogenetically along with 14 reported HEV isolates,The data showed that HEV in swine in Daqing was genetically closely related to human HEV genotype IV.The full length sequence of HEV DQ1 showed identities of 82.6 % to 83.6 %?87.0 % to 88.4 %?94.4 % to 96.5 % with HEV genotype IV in ORF1?ORF2?ORF3,respectively.ORF1 protein of HEV DQ1 was 93.5 % identical to that of HEV genotype IV.ORF2 and ORF3 protein of HEV DQ1 were 95.8 % to 97.4% and 90.3 % to 96.5 % identical respectively to that of HEV genotype IV.These findings suggest that swine HEV DQ1 comes from HEV genotype IV. |
| Keywords: | swine hepatitis E virus cloning sequence analysis |
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