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| 不同源性H5亚型禽流感病毒株HA基因序列分析 | |
| 引用本文： | 王伟利,肖成蕊,周宇,刘明,钱爱东.不同源性H5亚型禽流感病毒株HA基因序列分析[J].中国预防兽医学报,2006,28(1):41-43. |
| 作者姓名： | 王伟利 肖成蕊 周宇 刘明 钱爱东 |
| 作者单位： | 1. 吉林出入境检验检疫局,吉林,长春,130062 2. 吉林农业大学,吉林,长春,130118 3. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
| 摘 要： | 本研究对1株鹅源和一株鸽源的H5N1亚型禽流感病毒血凝素（HA）基因进行扩增、克隆和序列分析。结果表明，所扩增的片段长均为1707bp，包含完整的开放阅读框架，编码568个氨基酸；该毒株有7个潜在糖基化位点；在HA裂解位点附近有6个碱性氨基酸序列插入。两毒株间核苷酸与氨基酸的同源性均为96．7％。该毒株与参考毒株的序列比较分析结果表明：核苷酸同源率分别为95．3％～99％；氨基酸同源率分别为95．1％～99.3％。 |
| 关 键 词： | 鹅 鸽 禽流感病毒 血凝素基因 序列分析 |
| 文章编号： | 1008-0589（2006）01-0041-03 |
| 收稿时间： | 2004-09-10 |
| 修稿时间： | 2004年9月10日 |
Comparing and analysis of HA Gene of H5 influenza A virus from various animal species |
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| WANG Wei-li,XIAO Cheng-rui,ZHOU Yu,LIU ming,QIAN Ai-dong.Comparing and analysis of HA Gene of H5 influenza A virus from various animal species[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(1):41-43. | |
| Authors: | WANG Wei-li XIAO Cheng-rui ZHOU Yu LIU ming QIAN Ai-dong |
| Abstract: | In this study,cDNAS of HA gene of A goose and A pigeon H5 avian influenza isolates were amplified and cloned and then sequenced.The results of sequence showed that 1 707 bp of two HA cDNAs covered the whole open reading frames encoding 468 amino acid residues; There are 7 potential glycosylation sites and they both share 6 basic amino acids insert at the cleavage site in the influenza virus isolates. There are high homology of 96.7 % between the two isolates.The results of comparative sequence analysis indicated that 95.3 % to 99 % of nucleotide homology and 95.1% to 99.3 % of amino acid homology with other reference strains. |
| Keywords: | goose pigeon avian influenza virus hemagglutinin gene sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |