| 二重RT-PCR同时检测VSV与BVDV核酸 |
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| 引用本文： | 杨桂梅,徐自忠,高洪,花群义,周晓黎,杨昌焰.二重RT-PCR同时检测VSV与BVDV核酸[J].中国预防兽医学报,2003,25(4):291-293. |
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| 作者姓名： | 杨桂梅 徐自忠 高洪 花群义 周晓黎 杨昌焰 |
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| 作者单位： | 1. 云南农业大学,动物科学技术学院,云南,昆明,650201
2. 云南出入境检验检疫局技术中心,云南,昆明,650228 |
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| 基金项目： | 云南省计委科技攻关项目资助课题 (编号 :云计科 2 5 3号 ) |
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| 摘 要： | 水泡性口炎病毒(VSV)与牛病毒性腹泻病毒(BVDV)具有相近的传播途径与类似的检测方法，本文参照文献报道的基因序列，设计合成了两对能分别扩增VSV(202bp)、BVDV(341bp)基因片段的引物，并对PCR扩增条件进行优化，建立了二重RT-PCR方法，可同时检测VSV与BVDV病毒核酸。VSV产物经测序显示与报道的核酸序列同源性为88．6％。二重RT-PCR同时检测VSV与BVDV经济、快速、敏感、特异，可用于实验研究和流行病学调查。
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| 关 键 词： | 二重聚合酶链反应 水泡性口炎病毒 牛病毒性腹泻病毒 |
| 文章编号： | 1008-0589(2003)04-0291-03 |
| 修稿时间： | 2002年12月9日 |
Detection of viral nucleic acid of vesicular stomatitis virus and bovine viral diarrhea virus simultaneously by Bi-PCR |
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| YANG Gui_mei ,XU Zi_zhong ,GAO Hong ,HUA Qun_yi ,ZHUO Xiao_li ,YANG Chang_yan.Detection of viral nucleic acid of vesicular stomatitis virus and bovine viral diarrhea virus simultaneously by Bi-PCR[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(4):291-293. |
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| Authors: | YANG Gui_mei XU Zi_zhong GAO Hong HUA Qun_yi ZHUO Xiao_li YANG Chang_yan |
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| Institution: | YANG Gui_mei 1,XU Zi_zhong 2,GAO Hong 1,HUA Qun_yi 2,ZHUO Xiao_li 2,YANG Chang_yan 2 |
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| Abstract: | Vesicular Stomatitis Virus(VSV) and Bovine Viral Diarrhea Virus(BVDV) have similar infections routes and detection methodologies.Two pairs of oligonucleotide primers were designed and synthesized according to VSV N gene and BVDV P 125 gene sequences reported that amplify products of 202 bp and 341 bp.The condtions for Bi_PCR were optimized,The Bi_PCR method for detection VSV and BVDV were established.Sequence analysis on the amplified products of VSV has showed a 88.6 % of nucleotide hemology conmparred with sequences reported by Rhodes,D.P.and Baner jee,A.K.The Bi_PCR appears to be a rapid,sensitive and specific method for detecting VSV and BVDV and may by applied for epidemiologically investigate and laboratory diagnosis. |
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| Keywords: | Bi_PCR vesicular stomatitis virus bovine viral diarrhea virus |
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