| 框镜鲤维氏气单胞菌CY0806株气溶素基因的生物信息学分析及原核表达 |
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| 引用本文： | 吴同垒,单晓枫,孟庆峰,郭伟生,王伟利,钱爱东.框镜鲤维氏气单胞菌CY0806株气溶素基因的生物信息学分析及原核表达[J].中国预防兽医学报,2011,33(11). |
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| 作者姓名： | 吴同垒 单晓枫 孟庆峰 郭伟生 王伟利 钱爱东 |
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| 作者单位： | 1. 吉林农业大学动物科学技术学院,吉林长春,130118
2. 吉林进出口检验检疫局,吉林长春,130062 |
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| 基金项目： | 国家支撑计划项目，吉林省科技厅项目，吉林农业大学青年启动基金 |
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| 摘 要： | 为深入研究维氏气单胞菌(Aeromonas veronii)毒力因子气溶素(aerA),本实验从框镜鲤A.veronii CY0806株中克隆aerA基因,进行同源性分析和构建系统进化树,并进行生物信息学分析、诱导表达和检测.结果表明aerA基因全长1 471bp,编码490个氨基酸,CY0806株aerA与A.veronii EF620533.1株同源性为95％,与EF620533.1、AB109093.1和ET034117.1在同一分支；推测其相对分子质量约为54.33ku,理论等电点为5.90,摩尔消光系数为138 810,半衰期为30 h(体外培养的哺乳动物网状细胞),不稳定性系数为31.87,脂肪系数为71.86,总平均疏水性为-0.445; aerA蛋白具有信号肽,不存在跨膜区,二级结构分析表明aerA蛋白结构比较复杂.本研究对aerA基因进行了原核表达,产物具有一定的免疫原性.研究结果为进一步研究A.veronii aerA的功能和诊断、防治奠定基础.
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| 关 键 词： | 维氏气单胞菌 气溶素 生物信息学分析 原核表达 |
Bioinformatic analysis and prokaryotic expression of Aerosysin geneof Aeromonas veronii isolated from Cyprinus carpio |
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| WU Tong-lei,SHAN Xiao-feng,MENG Qing-feng,GUO Wei-sheng,WANG Wei-li,QIAN Ai-dong.Bioinformatic analysis and prokaryotic expression of Aerosysin geneof Aeromonas veronii isolated from Cyprinus carpio[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11). |
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| Authors: | WU Tong-lei SHAN Xiao-feng MENG Qing-feng GUO Wei-sheng WANG Wei-li QIAN Ai-dong |
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| Institution: | WU Tong-lei1#,SHAN Xiao-feng1#,MENG Qing-feng2,GUO Wei-sheng1,WANG Wei-li2,QIAN Ai-dong1(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,2.Jilin Entry-Exit Inspection and Quarantine Bureau,Changchun 130062,China) |
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| Abstract: | In this experiment,the aerA gene was amplaified from Aeromonas veronii CY0806 isolated from Cyprinus carpio by PCR,and expressed in BL21(DE3).The results showed that the length of aerA gene was 1 471 bp coding 490 amino acid residues,.The aerA gene of CY0806 was 95% in homology with A.veronii(EF620533.1) and was clustered into the same branch with A.veronii(EF620533.1,AB109093.1 and A.veronii).The molecular weight,theoretical pI,Ext.coefficient,half-life,instability index,aliphatic index and grand average o... |
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| Keywords: | Aeromonas veronii aerA bioinformatic analysis prokaryotic expression |
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