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鸡骨髓源树突状细胞的诱导分化及鉴定
引用本文:李冉,王桂军,王汉清,赵圆圆,朱英奇,王蓓,孙裴,秦爱建.鸡骨髓源树突状细胞的诱导分化及鉴定[J].中国预防兽医学报,2011,33(9):742-745.
作者姓名:李冉  王桂军  王汉清  赵圆圆  朱英奇  王蓓  孙裴  秦爱建
作者单位:1. 安徽农业大学动物科技学院,安徽合肥,230036
2. 扬州大学兽医学院,江苏扬州,225009
基金项目:农业公益性行业科研专项(20080319); 安徽农业大学第七批大学生创新基金资助
摘    要:为建立鸡骨髓源树突状细胞(Dcs)的体外培养和鉴定方法,本研究无菌抽取10日龄健康SPF雏鸡的骨髓,体外分离纯化骨髓细胞,将其培养于含有重组的人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和白细胞介素4 (rhIL4)的RPMI 1640营养液中,诱导培养鸡骨髓源DCs.采用显微镜观察其体外培养过程中的形态特征及流...

关 键 词:鸡骨髓细胞  树突状细胞  体外培养  鉴定

The development of chicken bone marrow-derived dendritic cells inducing with cytokines in vitro
LI Ran,Wang Gui-Jun,WANG Han-qing,ZHAO Yuan-yuan,ZHU Ying-qi,WANG Bei,SUN Pei,QIN Ai-jian.The development of chicken bone marrow-derived dendritic cells inducing with cytokines in vitro[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(9):742-745.
Authors:LI Ran  Wang Gui-Jun  WANG Han-qing  ZHAO Yuan-yuan  ZHU Ying-qi  WANG Bei  SUN Pei  QIN Ai-jian
Institution:LI Ran1,Wang Gui-Jun1,WANG Han-qing1,ZHAO Yuan-yuan1,ZHU Ying-qi1,WANG Bei1,SUN Pei1,QIN Ai-jian2(1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China,2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China)
Abstract:To explore the methods of inducing dendritic cells(DCs) from chicken bone marrow cells in vitro,chicken bone marrow cells were taken from 10-day-old chicken and cultured in the presence of recombinant human granulocyte-macrophage factor(rhGM-CSF) and rhIL-4.The results demonstrated that the cells showed typical morphologic and phenotypic characteristics of DCs after 8 day's cultivation and expressed higher level of CD11c as the surface marker of DCs detected by FACS staining with PE-conjugated anti-CD11c an...
Keywords:chicken bone marrow  dendritic cells  culture in vitro  identification  
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