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斑点免疫金渗滤法检测鸡白痢/鸡伤寒沙门氏菌抗体的应用研究
引用本文:曹春梅,焦新安,欧卫军,曹志刚,潘志明,刘秀梵.斑点免疫金渗滤法检测鸡白痢/鸡伤寒沙门氏菌抗体的应用研究[J].中国预防兽医学报,2006,28(5):577-580.
作者姓名:曹春梅  焦新安  欧卫军  曹志刚  潘志明  刘秀梵
作者单位:1. 扬州大学,江苏,扬州,225009
2. 南通伊士生物技术公司,江苏,南通,226009
基金项目:国家自然科学基金;江苏省六大人才高峰基金
摘    要:将鸡伤寒沙门氏菌LPS抗原包被于渗滤盒检测区(T区),针对沙门氏菌O9抗原单克隆抗体3-47-0包被在质控区(C区),用胶体金标记鸡伤寒沙门氏菌LPS抗原,建立了一种抗原夹心法快速检测鸡白痢、鸡伤寒沙门氏菌抗体的斑点免疫金渗滤法(DIGFA)。DIGFA比玻板凝集试验(PAT)灵敏度高,人工感染鸡试验表明在感染后第7d即可从32只鸡中的7只检测到抗体,在时间上早于PAT。722份现场血清样品的检疫结果表明,DIGFA的阳性检出率稍高于PAT,阳性样品抗体几何平均滴度DIGFA大于PAT法(P〈0.05)。DIGFA的结果得到细菌分离试验的验证。这些结果表明DIGFA快速、灵敏、准确,为鸡伤寒和鸡白痢的检疫提供了一个新的有效手段。

关 键 词:鸡白痢/鸡伤寒沙门氏菌  斑点免疫金渗滤法(DIGFA)
文章编号:1008-0589(2006)05-0577-04
收稿时间:2005-05-18
修稿时间:2005年5月18日

Dot immuno-gold filtration assay to detect the antibodies against Salmonella pullorum/gallinarum
CAO Chun-mei,JIAO Xin-an,OU Wei-jun,CAO Zhi-gang,PAN Zhi-ming,LIU Xiu-fan.Dot immuno-gold filtration assay to detect the antibodies against Salmonella pullorum/gallinarum[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(5):577-580.
Authors:CAO Chun-mei  JIAO Xin-an  OU Wei-jun  CAO Zhi-gang  PAN Zhi-ming  LIU Xiu-fan
Institution:1 .Yangzhou University, Yangzhou 225009, China; 2. Nantong Yishi Bioteehnology Company, Nantong 226009, China
Abstract:Dot immuno-gold filtration assay(DIGFA)to detect antibodies against Salmonella pullorum/gallinarum was established by using lipopolysaccharide(LPS)from Salmonella gallinarum reference strain as coating and labeling antigen.DIGFA was more sensitive than two commercially available plate agglutination test(PAT)reagents,as shown by positive sample dilution experiment and artificial infection experiment.722 samples were detected by DIGFA and two PAT reagents,the results showed DIGFA was more sensitive,which was further demonstrated by the positive serum antibody arithmetical average titer.The result of DIGFA was confirmed by bacterial isolation and identification.In summary,DIGFA is rapid,sensitive,specific,which offers a new method to screen fowl pullorosis and typhoid disease in chickens.
Keywords:Salmonella pullorum/gallinarum  dot immuno-gold filtration assay(DIGFA)
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