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| 新Ⅰ型鸭肝炎病毒VP1和3D蛋白的原核表达及鉴定 | |
| 引用本文： | 孟凡依,殷秀辰,李刚,陈玉环,张云,刘明,冯新畅.新Ⅰ型鸭肝炎病毒VP1和3D蛋白的原核表达及鉴定[J].中国预防兽医学报,2012,34(2):149-151. |
| 作者姓名： | 孟凡依 殷秀辰 李刚 陈玉环 张云 刘明 冯新畅 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所动物细菌病研究室,黑龙江哈尔滨150001;东北农业大学动物医学学院,黑龙江哈尔滨150030 2. 中国农业科学院哈尔滨兽医研究所动物细菌病研究室,黑龙江哈尔滨,150001 3. 东北农业大学动物医学学院,黑龙江哈尔滨,150030 |
| 基金项目： | 现代农业产业技术水禽体系岗位科学家专项(CARS-43-10) |
| 摘 要： | 为表达新Ⅰ型亚肝炎病毒(DHV-1C)的VP1和3D重组蛋白,本研究根据GenBank中的DHV-1C N株全基因序列,设计合成两对引物扩增其VP1和3D基因,构建重组表达载体并进行诱导表达.SDS-PAGE和western blot分析表明,VP1和3D重组蛋白分别在诱导3h和4h后表达量达到峰值,其大小分别为37.68 ku和65.80 ku,并且能够与抗DHV-1C阳性血清产生特异性免疫反应. |
| 关 键 词： | 新Ⅰ型鸭肝炎病毒 DHV-1C 3D VP1 克隆表达 |
Expression and identification of VP1-/3D-encoding genes of new duck hepatitis type 1 virus |
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| MENG Fan-yi , YIN Xiu-chen , LI Gang , CHEN Yu-huan , ZHANG Yun , LIU Ming , FENG Xin-chang.Expression and identification of VP1-/3D-encoding genes of new duck hepatitis type 1 virus[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(2):149-151. | |
| Authors: | MENG Fan-yi YIN Xiu-chen LI Gang CHEN Yu-huan ZHANG Yun LIU Ming FENG Xin-chang |
| Institution: | 1.Division of Bacterial Diseases,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China; 2.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: | To express the VP1-/3D protein of new duck hepatitis type 1 virus(DHV-1C),the VP1-/3D-encoding genes were amplified by PCR with primers designed according to DHV-1C N strain and cloned into expression vector pET-30a,respectively.The recombinant VP1 and 3D were highly expressed for 3 hours and 4 hours after IPTG induction,respectively.SDS-PAGE analysis showed that the VP1 and 3D products were 37 ku and 57 ku,respectively.Western blot demonstrated that VP1 and 3D proteins reacted specifically with duck anti DHV-1C serum.The expression of VP1 and 3D protein in bacterial could be useful in the development of diagnostic kit and subunit vaccine,or the study of pathological mechanism of DHV-1C. |
| Keywords: | new duck hepatitis type 1 virus DHV-1C 3D VP1 clone and expression |
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