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| 犬细小病毒环介导等温扩增快速检测方法的初步建立 | |
| 引用本文： | 张坤,马丽娟,张乃生,李刚,邢国栋,史利军,李伟.犬细小病毒环介导等温扩增快速检测方法的初步建立[J].中国预防兽医学报,2010,32(4). |
| 作者姓名： | 张坤 马丽娟 张乃生 李刚 邢国栋 史利军 李伟 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,动物营养国家重点实验室,北京,100193 2. 吉林大学畜牧兽医学院,长春,130062 3. 昌平区动物疫病预防控制中心,北京,102200 |
| 基金项目： | 国家高技术研究发展计划(863计划)，中央级公益性科研院所基本业务费专项，北京市科学技术委员会科研基金 |
| 摘 要： | 为建立犬细小病毒(CPV)环介导等温扩增(LAMP)检测方法,实现CPV的早期快速诊断,本研究根据GenBank登录的CPV VP2基因序列,在其序列保守区域设计LAMP引物,利用CPV基因组DNA为模板进行扩增。结果表明:LAMP方法检测灵敏度达到10-1TCID50/mL;并且与其它细小病毒等无特异性扩增,表现出良好的特异性。与PCR技术相比,LAMP法操作更加简单方便,更适合基层和实验室的快速检测。 |
| 关 键 词： | 犬细小病毒 环介导等温扩增 条件优化 |
Rapid detction of canine parvovirus by loop-mediated isothermal amplification method |
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| ZHANG Kun,MA Li-juan,ZHANG Nai-sheng,LI Gang,XING Guo-dong,SHI Li-jun,LI Wei.Rapid detction of canine parvovirus by loop-mediated isothermal amplification method[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(4). | |
| Authors: | ZHANG Kun MA Li-juan ZHANG Nai-sheng LI Gang XING Guo-dong SHI Li-jun LI Wei |
| Institution: | ZHANG Kun1,a,MA Li-juan2,ZHANG Nai-sheng,LI Gang1,XING Guo-dong3,SHI Li-jun1,LI Wei1(1.State Key Laboratory of Animal Nutrition,Beijing Institute of Animal Science , Veterinary Medicine,Chinese Academy of Agricultural Sciences,Beijing 100193,China,2. College of Veterinary Medicine,Jilin University,Changchun 130062,3. Changping Center for Animal Disease Control , Prevention,Beijing 102200,China) |
| Abstract: | A loop-mediated isothermal amplification assay(LAMP) for rapid and accurate detection of canine parvovirus(CPV) was developed using specific primers derived from VP2 gene of CPV. The assay was specific for CPV and did not amplify any PCR product from porcine parvovirus,gosling plague virus,canine distemper virus. It was highly sensitive and had a detection limit of 2 to 3 DNA copies. The LAMP assay was simple and more convenient than PCR method,which could provide a useful technique for CPV clinic sample de... |
| Keywords: | Canine Parvovirus LAMP condition optimization |
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