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中国地方品种丝羽乌骨鸡白细胞介素2基因的克隆及遗传进化分析
引用本文:陈吉刚,周继勇,郑肖娟.中国地方品种丝羽乌骨鸡白细胞介素2基因的克隆及遗传进化分析[J].中国预防兽医学报,2003,25(1):21-23.
作者姓名:陈吉刚  周继勇  郑肖娟
作者单位:浙江大学,动物预防医学研究所,浙江,杭州,310029
基金项目:杭州市科委重大攻关项目
摘    要:鸡白细胞介素2(ChIL-2)是近年来新发现的一类细胞因子。根据Sundick等发表的鸡IL-2基因序列设计一对特异性引物,从ConA体外激活的脾脏淋巴细胞中提取mRNA,通过RT-PCR方法扩增和克隆了我国地方品种丝羽乌骨鸡IL-2cDNA,丝羽乌骨鸡IL-2基因由734nt组成,编码-由143个氨基酸组成的前体蛋白,基因5’端含有17个核苷酸和3'端含有285个核苷酸组成的非编码区,3'UTR中含有4个重复的“ATTTA”序列。编码蛋白氨基酸与Kestrel,Obese和SC品系的来杭鸡比较,氨基酸的突变主要发生在28-32位,这一区域丝羽乌骨鸡和Kestrel来杭鸡相同,Obese和SC鸡相同,基因系统进化树分析表明丝羽乌骨鸡和Kestrel来杭鸡具有很近的亲缘关系,Lbese和SC鸡具有很近的亲缘关系。中国的药用鸡品种-丝羽乌骨鸡在非常保守的133位发生了氨基酸突变,我们推测这可能与该品种鸡具有很强的抗病能力有关。

关 键 词:中国  地方品种  丝羽乌骨鸡  白细胞介素2  遗传进化  基因克隆  细胞因子  免疫佐剂
文章编号:1008-0589(2003)01-0021-03
修稿时间:2002年6月22日

Cloning and Genetic Analysis of Interleukin 2 Gene in Silken Feather Silky Chicken of Chinese Local Breed
CHEN Ji-gang,ZHOU Ji-yong,ZHENG Xiao-juan.Cloning and Genetic Analysis of Interleukin 2 Gene in Silken Feather Silky Chicken of Chinese Local Breed[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(1):21-23.
Authors:CHEN Ji-gang  ZHOU Ji-yong  ZHENG Xiao-juan
Abstract:Chicken interleukin-2(ChIL-2)is a kind of important cytokine discovered recently.One pair of sepcific primers were designed according to IL-2 gene sequence published by Sundick,et al,IL-2 gene of silken feather Silky chicken was cloned by RT-PCR method and mRNA were extracted from spleen lymphocytes activated by ConA.Sequencing results showed that the gene was 734bp in length,encoding a preprotein of 143aa residues.There were an URT of 17 nucleotides at 5'-terminal of the gene of an UTR of 285 nucleotides along with 4 repetitive sequences of " ATTTA " at 3' terminal of the gene.Compared with that of leghorn(Kestrel,Obese and SC),the deduced amino acids were predominantly mutated at the region of 28-32.The sequence of this region were entirely conserved between Silky and Kestrel,and also between Obese and SC.Phylogenetic analysis showed there are close relationships between Silky and Kestrel,and also between Obese and SC.Amino acids nutation were found at the region of 133 of Chinese officinal chicken breed-silken feather Silky chicken,which were highly conserved usually,we sepculated that this may be related with its high resistence against sickness.
Keywords:ChIL-2  Cloning  Phylogenetic analysis
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