| 裸鼠过度角化症牛棒状杆菌的分离鉴定 |
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| 引用本文： | 张强,熊炜,李健,李树清,王巧全,黄忠荣,陈粹.裸鼠过度角化症牛棒状杆菌的分离鉴定[J].中国预防兽医学报,2011,33(8). |
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| 作者姓名： | 张强 熊炜 李健 李树清 王巧全 黄忠荣 陈粹 |
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| 作者单位： | 上海出入境检验检疫局,上海,200135 |
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| 基金项目： | 上海检验检疫局科技项目(沪科08-02) |
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| 摘 要： | 2010年8月一批进口入境的裸鼠在隔离期间发生了疑似国外文献报道的过度角化性皮炎,为了确诊该病及鉴定该病的病原,本研究从患病裸鼠皮肤组织分离到一株革兰氏阳性杆菌。细菌纯化后经Biolog自动生物鉴定仪系统初步鉴定为牛棒状杆菌(C.bovis);提取细菌基因组,PCR扩增16S rRNA基因,测序结果经比对与GenBank中的C.bovis同源性在99%以上;培养物感染裸鼠没有出现临床症状,但组织学检查显示,感染裸鼠表皮棘皮层增厚,表现出轻微的C.bovis感染特有的棘皮症。由此可以确认这次入境裸鼠暴发的疫病为过度角化症,病原为C.bovis。本研究首次报道了在我国实验动物设施暴发的裸鼠过度角化症,提示裸鼠感染C.bovis导致的过度角化症是实验动物质量监测中不可忽视的传染病。
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| 关 键 词： | 裸鼠 过度角化症 病原分离鉴定 牛棒状杆菌 |
Isolation and identification of Corynebacterium bovis of hyperkeratosis in athymic nude mice |
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| ZHANG Qiang,XIONG Wei,LI Jian,LI Shu-qing,WANG Qiao-quan,HUANG Zhong-rong,CHEN Cui.Isolation and identification of Corynebacterium bovis of hyperkeratosis in athymic nude mice[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(8). |
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| Authors: | ZHANG Qiang XIONG Wei LI Jian LI Shu-qing WANG Qiao-quan HUANG Zhong-rong CHEN Cui |
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| Institution: | ZHANG Qiang*,XIONG Wei,LI Jian,LI Shu-qing,WANG Qiao-quan,HUANG Zhong-rong,CHEN Cui(Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China) |
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| Abstract: | An outbreak of hyperkeratosis occurred in a batch of import athymic nude mice in a laboratory animal facility during quarantine period.A bacterium was isolated from the affected animals and the isolate was identificated as Corynebacterium bovis using Biolog Microbial Identification System and confirmed by the 16S rRNA gene sequencing.The 16S rRNA gene sequence of the isolate showed over 99% homology with the sequences of C.bovis reference strains in GenBank.Animal experimental infection also demonstated tha... |
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| Keywords: | Corynebacterium bovis athymic nude mice hyperkeratosis isolation and identification |
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