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长角血蜱肠道细菌的分离与鉴定
引用本文:李春红,周勇志,赵素华,刘毅,张厚双,曹杰,周金林.长角血蜱肠道细菌的分离与鉴定[J].中国预防兽医学报,2011,33(8).
作者姓名:李春红  周勇志  赵素华  刘毅  张厚双  曹杰  周金林
作者单位:1. 湖南农业大学动物医学院,湖南长沙410128;中国农业科学院上海兽医研究所,上海200241
2. 中国农业科学院上海兽医研究所,上海,200241
3. 宿州职业技术学院,安徽宿州,234101
4. 湖南农业大学动物医学院,湖南长沙,410128
基金项目:国家自然科学基金(30871846)
摘    要:为深入研究肠道细菌在蜱的生理学和传播病原上的作用,本研究进行了长角血蜱肠道细菌的分离鉴定。按照细菌传统分离方法并结合16S rDNA测序鉴定,获得7个分离株,这些菌株分别属于显核菌属(Caryophanon sp.)、考克氏菌属(Kocuria sp.)、葡萄球菌属(Staphylococcus sp.)、微杆菌属(Microbacterium sp.)和短杆菌属(Brevibacterium sp.)5个菌属。

关 键 词:长角血蜱  肠道细菌  系统分类法鉴定  16S  rDNA  

Isolation and identification of intestinal bacteria of Haemaphysalis longicornis
LI Chun-hong,ZHOU Yong-zhi,ZHAO Su-hua,LIU Yi,ZHANG Hou-shuang,CAO Jie,ZHOU Jin-lin.Isolation and identification of intestinal bacteria of Haemaphysalis longicornis[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(8).
Authors:LI Chun-hong  ZHOU Yong-zhi  ZHAO Su-hua  LIU Yi  ZHANG Hou-shuang  CAO Jie  ZHOU Jin-lin
Institution:LI Chun-hong1,2,ZHOU Yong-zhi2,ZHAO Su-hua3,LIU Yi1,ZHANG Hou-shuang2,CAO Jie2,ZHOU Jin-lin2*(1.College of Animal Medical,Hunan Agricultural University,Changsha 410128,China,2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Science,Shanghai 200241,3.College of Vocational and Technical of Suzhou,Suzhou 234101,China)
Abstract:The hard tick Haemaphysalis longicornis is an exclusively hematophagous ectoparasites of mammals,which is regarded as one of vectors for disease spread.To study the effect of intestinal bacteria on the physiology of tick and transmission of pathogen by ticks,seven intestinal bacteria were isolated from tick H.longicornis.These bacteria were identified by 16S rDNA sequencing as fine genera,Caryophanon sp.,Kocuria sp.,Staphylococcus sp.,Microbacterium sp.and Brevibacterium sp.
Keywords:Haemaphysalis longicornis  intestinal bacteria  systematic identification  16S rDNA  
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