RT-PCR检测和区分猪传染性胃肠炎病毒和猪呼吸道冠状病毒的研究

根据猪传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）的基因组核苷酸序列，在S基因（纤突蛋白基因）5′端保守区设计了一对引物P3／P4，该对引物在TGEV扩增跨幅约为2.4kb；而PRCV由于在此区域存在一约0.6kb碱基缺失，扩增跨幅约为1.8kb。用引物P3／P4对TGEV Miller株、Purdue株和PRCV AR310株分别进行RT－PCR，根据RT－PCR扩增片段大小可以直接区分TGEV和PRCV。用引物P3／P4与引物P1／P2作Nested-PCR,提高了该RT－PCR的特异性和敏感性，建立的RT－PCR可为临床上诊断TGEV及调查我国是否存在PRCV感染提供可靠的鉴别手段。

Detection and Differentiation of Transmissible Gastroentevitis Virus from Porcine Respiratory Coronavirus by RT-PCR

According to the published sequence of TEGV and PRCV,one pair of specific primers were designed as P 3/P 4 from S gene of TGEV and PRCV.The region between the primers were about 2.4kb of TGEV,and 1.8kb of PRCV,which PRCV has an deletion 0.6kb on s gene.The Pair of primers P 3/P 4 was used to detect 3 reference strains (TGEV Miller strain,purdue strain and PRCV AR310 strain) by RT_PCR.All strains Produced the predicted products.Based on the size of RT_PCR products,TGEV and PRCV could be quickly and easily discriminated. Nested_PCR with Primers P 3/P 4 and P 1/P 2 could improve the specificity and sensitivity.The RT_PCR may be used to detect TGEV and PRCV and distinguish them.