| 猪圆环病毒多重PCR诊断方法的建立 |
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| 引用本文： | 崔尚金,全滟平,李曦,符芳,姜艳玲,张莉.猪圆环病毒多重PCR诊断方法的建立[J].中国预防兽医学报,2006,28(5):581-584. |
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| 作者姓名： | 崔尚金 全滟平 李曦 符芳 姜艳玲 张莉 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,疫病诊断与流行病学中心/兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001
2. 北京市农林科学院,畜牧兽医研究所,北京,100097 |
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| 摘 要： | 根据GenBank中20株猪圆环病毒（PCV2）核苷酸序列设计两对引物，建立的检测猪圆环病毒的多重PCR方法，其中引物P1、P2为PCV特异性，扩增938bp片段。P3、P4为PCV2特异性扩增490bp，因此本方法不仅可以扩增PCV片段，还可以同时区分PCV1和PCV2。该方法具有良好的特异性和敏感性，为猪圆环病毒的临床诊断与流行病学调查等研究奠定了基础。
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| 关 键 词： | 猪圆环病毒 多重PCR |
| 文章编号： | 1008-0589(2006)04-0581-04 |
| 收稿时间： | 2004-11-16 |
| 修稿时间： | 2004年11月16 |
The establishment of a multiplex PCR of porcine circovirus |
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| CUI Shang-jin,QUAN Yan-ping,LI Xi,FU Fang,JIANG Yan-ling,ZHANG Li.The establishment of a multiplex PCR of porcine circovirus[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(5):581-584. |
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| Authors: | CUI Shang-jin QUAN Yan-ping LI Xi FU Fang JIANG Yan-ling ZHANG Li |
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| Institution: | 1. National Animal Disease Diagnosis and Epidemic Center, National key Laboratory of veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001,China; 2. Animal Hhusbandry and Veterinary Research Institute, Beijing Municipal Academy of Agriculture and Forestry, Beijing 100097,China |
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| Abstract: | The nuclear sequence homology and diversity of 20 strains of PCVI and PCV2 are compared to design two pairs of type-specific primers. The primers of Pl and P2 amplified the PCV virus-specific segment with the size of 938 bp. The primers of P3 and P4 amplified the PCV2 type-specific segment with the size of 490 bp. A multiplex PCR is developed based on these two pairs of primers to type and differentiate PCVl and PCV2.The optimization of the PCR reaction make the method more efficiently. The technique has been applied into the clinical diagnosis and epidemiological research. |
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| Keywords: | porcine circovirus multiplex PCR |
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