| 血红素加氧酶1对猪瘟病毒复制的影响 |
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| 引用本文： | 史子学,杨知,曲会,李江南,朱妍,郭焕成,涂长春.血红素加氧酶1对猪瘟病毒复制的影响[J].中国预防兽医学报,2010,32(4). |
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| 作者姓名： | 史子学 杨知 曲会 李江南 朱妍 郭焕成 涂长春 |
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| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062;军事医学科学院,军事兽医研究所,吉林,长春,130062
2. 军事医学科学院,军事兽医研究所,吉林,长春,130062 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家科技支撑计划 |
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| 摘 要： | 本实验研究了猪血红素加氧酶1对猪瘟病毒(CSFV)在PK-15细胞中复制的影响,本研究应用siRNA干扰方法抑制PK-15细胞中血红素加氧酶1表达后再接种CSFV,细胞中CSFV基因组增殖与对照组相比显著下降;感染病毒细胞中和培养上清中CSFV滴度也显著降低。本实验首次报道了干扰细胞中HO-1表达对CSFV细胞中增殖的影响,提出了CSFV与血红素加氧酶1新的相互作用关系。
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| 关 键 词： | 猪瘟病毒 血红素加氧酶1 病毒/细胞相互作用 复制 |
Heme oxygenase 1:a novel interaction protein involved in the replication of classical swine fever virus in PK-15 cells |
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| SHI Zi-xue,YANG Zhi,QU Hui,LI Jiang-nan,ZHU Yan,GUO Huan-cheng,TU Chang-chun.Heme oxygenase 1:a novel interaction protein involved in the replication of classical swine fever virus in PK-15 cells[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(4). |
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| Authors: | SHI Zi-xue YANG Zhi QU Hui LI Jiang-nan ZHU Yan GUO Huan-cheng TU Chang-chun |
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| Institution: | SHI Zi-xue1,2,YANG Zhi1,QU Hui2,LI Jiang-nan2,ZHU Yan2,GUO Huan-cheng2,TU Chang-chun2(1. College of Animal Science , Veterinary Medicine,Jilin University,Changchun 130062,China,2. Institute of Veterinary Sciences,Academy of Military Medical Sciences,China) |
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| Abstract: | In this study we defined heme oxygenase 1(HO-1) as a novel interaction partner of Classical swine fever virus(CSFV) in PK-15 cells. To test the functional relevance of HO-1 in CSFV replication,we examined viral replication in PK-15 cells where HO-1 expression had been decreased by siRNA methodology. PK-15 cells were treated with an HO-1-specific siRNA(siHO-1) and followed by inoculation with CSFV. HO-1 depletion in the PK-15 cells led to a significant decline in the viral replication and titers of released ... |
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| Keywords: | CSFV heme oxygenase 1 virus-cell interaction replication |
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