2011年猪流行性腹泻病毒的遗传变异分析

为分析猪流行性腹泻病毒(PEDV)的遗传变异情况,本研究设计合成2对扩增S1基因的引物,利用套式RT-PCR方法,对2011年分离的17个PEDV S1基因进行扩增、克隆和序列测定;并将其进行比对和遗传演化分析。结果表明:17个PEDV S1基因序列与参考病毒株S1基因核苷酸序列同源性为90.54%～99.96%,与经典病毒株CV777相比,其中有6个S1基因在453 bp～454 bp处存在3个核苷酸的缺失;一个在453 bp～454 bp处缺失6个核苷酸;其它10个S1基因在173 bp～186 bp之间存在12个核苷酸的插入,413 bp～417 bp之间有3个核苷酸的插入,467 bp～468 bp处缺失6个核苷酸,插入和缺失位点与韩国病毒株KNU-0901、KNU-0905、KNU-0801相似。S1基因系统进化树分析表明,PEDV S1基因分为3群,其中7个S1基因属于PEDVⅠ群,另外10个属于PEDVⅢ群。

Genetic variation analysis of porcine epidemic diarrhea virus isolated in 2011

Porcine epidemic diarrhea virus(PEDV) causes an acute and highly contagious enteric disease in swine.To investigate the variation in S1 gene of PEDV,a total of 17 PEDV S1 genes were cloned and sequenced from different swine breeding farms in different provinces in 2011.Compared with CV777,there were 3 nucleotides deletion between 453 bp to 454 bp in 6 isolates;There were 6 nucleotide deletion between 453 bp to 454 bp in one isolate.There were 12 nucleotide insertions between173 bp to 186 bp,3 nucleotide insertions between 413 bp to 417 bp and 6 nucleotide deletion between 467 bp to 468 bp,respectively,in the other 10 isolates.The nucleotide insertion and deletion were similar to KNU-0901,KNU-0905,KNU-0801 strain isolated in Korea.Sequence analysis showed that S1 gene shared 90.54% to 99.96% nucleotide identities with PEDV reference sequences.Phylogenetic analysis revealed that 7 of PEDV S1 gene belong to the first group.Other 10 of PEDV S1 gene belong to the third group.