| 抗牛布鲁氏菌独特型抗体杂交瘤细胞（Ｆ９）的生长和代谢 |
| |
| 引用本文： | 黄炯,黄丽茜,王学辉,谷登峰,麻迎东.抗牛布鲁氏菌独特型抗体杂交瘤细胞（Ｆ９）的生长和代谢[J].中国预防兽医学报,2001,23(1):11-13. |
| |
| 作者姓名： | 黄炯 黄丽茜 王学辉 谷登峰 麻迎东 |
| |
| 作者单位： | 1. 新疆畜牧科学院兽医研究所,
2. 新疆动物防疫监督总站, |
| |
| 基金项目： | 由上海华东理工大学生物反应器工程国家重点实验室开放课题资助 |
| |
| 摘 要： | 用RPMI1640培养基对抗牛布鲁氏菌独特型抗体杂交瘤细胞株(F9)进行培养，并对其生长代谢进行测定。该细胞不论接种1.1×10
|
| 关 键 词： | F9杂交瘤细胞 生长 代谢 抗独特型抗体 牛布鲁氏菌病 |
| 文章编号： | 1008-0589(2001)01-0011-03 |
| 修稿时间： | 2000年7月11日 |
Growth and Metabolism of
Hybridoma Cell(F9)of Anti-Bovine Brucellosis Idiotypic Antibody |
| |
| HUNAG Jiong,HUNAG Liqian,WHAG Xuehui,Gu Dengfeng,MA Yingdong.Growth and Metabolism of
Hybridoma Cell(F9)of Anti-Bovine Brucellosis Idiotypic Antibody[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(1):11-13. |
| |
| Authors: | HUNAG Jiong HUNAG Liqian WHAG Xuehui Gu Dengfeng MA Yingdong |
| |
| Institution: | HUNAG Jiong 1,HUNAG Liqian 1,WHAG Xuehui 1,MA Yingdong 2,GU Dengfeng 1 |
| |
| Abstract: | A hybridoma cell line(F9),which secreted anti_bovine brucellosis idiotypic antibody,was cultured with RPMI medium 1640 and then determined its growth and metabolism.Inoculating the cell with 1.1×105 cells/ml and 2.2×105 cells/ml,its highest output could only increase 2～3 times［3.22×105 cells/ml(48h)3.7×105 cells/ml(48h)］.Glucose was depleted in 48～72 hours and meanwhile produced a mass of lactic acid［1.48g/L(72h)and 1.65g/L(48h)］.With using up glucose,disappearing of carbon sources and accumulating lactic acid,the pH of medium sharply declined.These factors affected on the cell viability and resulted in the cells died immediately,hardly without maintain period. |
| |
| Keywords: | F9 hybridoma cell Growth and metabolism Anti_idiotypic antibody Bovine brucellosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
