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| 牛副结核分枝杆菌LAMP快速检测方法的建立 | |
| 引用本文： | 王建峰,黄素文,刘思国,倪建波,王春,张吉红,于纪棉,朱堂明,黄绍棠.牛副结核分枝杆菌LAMP快速检测方法的建立[J].中国预防兽医学报,2012,34(7):555-557. |
| 作者姓名： | 王建峰 黄素文 刘思国 倪建波 王春 张吉红 于纪棉 朱堂明 黄绍棠 |
| 作者单位： | 1. 宁波出入境检验检疫局,浙江宁波,315012 2. 中国农科院哈尔滨兽医研究所,黑龙江哈尔滨,150001 3. 宁波天一职业技术学院,浙江宁波,315000 |
| 基金项目： | 宁波市社会发展项目，国家质检总局科技计划项目 |
| 摘 要： | 为建立牛副结核分枝杆菌(MAP)的快速检测方法,本研究采用环介导等温扩增(LAMP)技术,以MAP的IS900基因序列为靶基因设计特异性引物,建立了MAP特异性的LAMP快速检测方法。经反应条件优化,检测结果显示,建立的LAMP检测方法具有良好的特异性,最低见测量为0.53 pg;与其他病原菌无任何交叉反应。临床样品检测与行业标准的符合率为100%。该LAMP检测方法可以用于MAP的常规检疫。 |
| 关 键 词： | 牛副结核分枝杆菌 环介导等温扩增 检测 |
Development of a LAMP assay for Mycobacterium avium subsp.Paratuberculosis |
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| WANG Jian-feng , HUANG Su-wen , LIU Si-guo , NI Jian-bo , WANG Chun , ZHANG Ji-hong , YU Ji-mian , ZHU Tang-ming , HUANG Shao-tang.Development of a LAMP assay for Mycobacterium avium subsp.Paratuberculosis[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(7):555-557. | |
| Authors: | WANG Jian-feng HUANG Su-wen LIU Si-guo NI Jian-bo WANG Chun ZHANG Ji-hong YU Ji-mian ZHU Tang-ming HUANG Shao-tang |
| Institution: | 1(1.Ningbo Entry-Exit Inspetion and Quarantine Bureau,Ningbo 315012,China;2.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China;3.Ningbo College of Health Sciences,Ningbo 315000,China) |
| Abstract: | To establish a quick detection protocol for Mycobacterium avium subsp.paratuberculosis(MAP),a loop-mediated isothermal amplification(LAMP) assay was developed with primers designed according to the IS900 gene of MAP available in GenBank.Under the optimized amplification conditions.The LAMP assay was specific to MAP with detection limit of 0.53 pg and no cross-reactions with other related bacteria.These results demonstrated that the LAMP assay is a useful technique for routine MAP quarantine. |
| Keywords: | Mycobacterium avium subsp paratuberculosis LAMP detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |