致肾脏、肠道病变大肠杆菌的分离及生物特性鉴定

利用常规培养基从病鸡的肾脏、肠道等组织器官中分离出了二株大肠杆菌，血清型为0152-160，该菌株能引起7／10的10日龄SPF鸡胚死亡，死亡鸡胚肾、肝肿大，全身出血。用分离物1^#、2^#大肠杆菌感染的20日龄雏鸡67％～88％死亡，从试验发病死亡鸡肾脏分离到的大肠杆菌仍能67％的感染鸡死亡。死亡鸡呈严重的肾肿大、尿酸盐沉积，肠壁出血、粘膜脱落，气囊炎和心包炎。该大肠杆菌对SPF鸡的致病死亡率低于普通健康鸡；试验证明用鸡毒支原体激发感染，可明显增强该大肠杆菌对SPF鸡的致死率和病变程度。另外试验证明此大肠杆菌对青、链霉素、庆大霉素等具有强耐药性。

Isolation and biological characterization on E. coli induced Nephropathy and enteritis of chicken

Two Escherichia coli(E.coli) 1#and2# were isolated from 20-day infected and dead chicken tissues.Both are O152-O160 seratype.70% of SPF chicken embryos were died within 120 hours after inoculated with these srtains.The liver and kidneyare swelled as well as whole bodyhaemorrhaged ofembryos.The 20-daychicken infect-ed with 1# or 2# E.coli strain cultures respectively 67%-88% of them were died post infected 22 days.Chicken in-fected with 2# E.coli strain that recovered fromkidneyofchicken artificial infected in laboratory67% died in 20 days.Gross Pathologyresults showed that the dead chick with serious kidneyswelled;intestines hemorrhahed and air-sac-titis were more severe than common health chicken also.The results showed that the pathogenicity of the E.coli is much more rirulent to SPF chicken with M.gallisepticum infected mit.The other results showed that these two E.coli strains have strongresistance topenivillin,streptomycin and gentamycin etc.